Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

10.1186/s12934-016-0540-5

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Main Authors:	Camattari, A, Goh, A, Yip, L.Y, Meng Tan, A.H, Ng, S.W, Tran, A, Liu, G, Liachko, I, Dunham, M.J, Rancati, G
Other Authors:	DEPT OF BIOCHEMISTRY
Format:	Article
Published:	2020
Subjects:	blue fluorescent protein fluorescent dye genomic DNA recombinant protein unclassified drug methanol Article controlled study DNA extraction expression vector fluorescence fluorescence activated cell sorting fungal genome fungal strain gene dosage genetic recombination genetic transformation Komagataella pastoris molecular cloning nonhuman panARS based episomal vector plasmid polymerase chain reaction reporter gene synthetic biology yeast cell biosynthesis gene expression regulation gene vector genetics metabolism Pichia procedures promoter region protein processing Cloning, Molecular Gene Expression Regulation, Fungal Genetic Vectors Methanol Plasmids Promoter Regions, Genetic Protein Processing, Post-Translational Recombinant Proteins Recombination, Genetic Synthetic Biology
Online Access:	https://scholarbank.nus.edu.sg/handle/10635/181404
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Institution:	National University of Singapore

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spelling	sg-nus-scholar.10635-1814042023-10-31T09:14:45Z Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications Camattari, A Goh, A Yip, L.Y Meng Tan, A.H Ng, S.W Tran, A Liu, G Liachko, I Dunham, M.J Rancati, G DEPT OF BIOCHEMISTRY blue fluorescent protein fluorescent dye genomic DNA recombinant protein unclassified drug methanol recombinant protein Article controlled study DNA extraction expression vector fluorescence fluorescence activated cell sorting fungal genome fungal strain gene dosage genetic recombination genetic transformation Komagataella pastoris molecular cloning nonhuman panARS based episomal vector plasmid polymerase chain reaction reporter gene synthetic biology yeast cell biosynthesis gene expression regulation gene vector genetic recombination genetics metabolism Pichia plasmid procedures promoter region protein processing synthetic biology Cloning, Molecular Gene Expression Regulation, Fungal Genetic Vectors Methanol Pichia Plasmids Promoter Regions, Genetic Protein Processing, Post-Translational Recombinant Proteins Recombination, Genetic Synthetic Biology 10.1186/s12934-016-0540-5 Microbial Cell Factories 15 1 139 2020-10-27T10:49:19Z 2020-10-27T10:49:19Z 2016 Article Camattari, A, Goh, A, Yip, L.Y, Meng Tan, A.H, Ng, S.W, Tran, A, Liu, G, Liachko, I, Dunham, M.J, Rancati, G (2016). Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications. Microbial Cell Factories 15 (1) : 139. ScholarBank@NUS Repository. https://doi.org/10.1186/s12934-016-0540-5 14752859 https://scholarbank.nus.edu.sg/handle/10635/181404 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ Unpaywall 20201031
institution	National University of Singapore
building	NUS Library
continent	Asia
country	Singapore Singapore
content_provider	NUS Library
collection	ScholarBank@NUS
topic	blue fluorescent protein fluorescent dye genomic DNA recombinant protein unclassified drug methanol recombinant protein Article controlled study DNA extraction expression vector fluorescence fluorescence activated cell sorting fungal genome fungal strain gene dosage genetic recombination genetic transformation Komagataella pastoris molecular cloning nonhuman panARS based episomal vector plasmid polymerase chain reaction reporter gene synthetic biology yeast cell biosynthesis gene expression regulation gene vector genetic recombination genetics metabolism Pichia plasmid procedures promoter region protein processing synthetic biology Cloning, Molecular Gene Expression Regulation, Fungal Genetic Vectors Methanol Pichia Plasmids Promoter Regions, Genetic Protein Processing, Post-Translational Recombinant Proteins Recombination, Genetic Synthetic Biology
spellingShingle	blue fluorescent protein fluorescent dye genomic DNA recombinant protein unclassified drug methanol recombinant protein Article controlled study DNA extraction expression vector fluorescence fluorescence activated cell sorting fungal genome fungal strain gene dosage genetic recombination genetic transformation Komagataella pastoris molecular cloning nonhuman panARS based episomal vector plasmid polymerase chain reaction reporter gene synthetic biology yeast cell biosynthesis gene expression regulation gene vector genetic recombination genetics metabolism Pichia plasmid procedures promoter region protein processing synthetic biology Cloning, Molecular Gene Expression Regulation, Fungal Genetic Vectors Methanol Pichia Plasmids Promoter Regions, Genetic Protein Processing, Post-Translational Recombinant Proteins Recombination, Genetic Synthetic Biology Camattari, A Goh, A Yip, L.Y Meng Tan, A.H Ng, S.W Tran, A Liu, G Liachko, I Dunham, M.J Rancati, G Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications
description	10.1186/s12934-016-0540-5
author2	DEPT OF BIOCHEMISTRY
author_facet	DEPT OF BIOCHEMISTRY Camattari, A Goh, A Yip, L.Y Meng Tan, A.H Ng, S.W Tran, A Liu, G Liachko, I Dunham, M.J Rancati, G
format	Article
author	Camattari, A Goh, A Yip, L.Y Meng Tan, A.H Ng, S.W Tran, A Liu, G Liachko, I Dunham, M.J Rancati, G
author_sort	Camattari, A
title	Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications
title_short	Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications
title_full	Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications
title_fullStr	Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications
title_full_unstemmed	Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications
title_sort	characterization of a panars-based episomal vector in the methylotrophic yeast pichia pastoris for recombinant protein production and synthetic biology applications
publishDate	2020
url	https://scholarbank.nus.edu.sg/handle/10635/181404
_version_	1781792502640017408

Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

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