Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells

• Main Authors: Chen, A, Poh, S.L, Dietzsch, C, Roethl, E, Yan, M.L, Ng, S.K
• Other Authors: PHARMACY
• Format: Article
• Published: 2020
• Subjects: Antiviral response; Batch bioreactors; Bioreactor system; Cell concentrations; Cell density; Clinical trial; Deletion mutants; Efficient production; Gene deletion; Health concerns; Human society; Influenza; Influenza vaccines; Influenza virus; Manufacturability; Microcarrier; Microcarrier culture; Microcarriers; Nonstructural proteins; NS1; Phase I; Production process; Serum-free media; Serum-free medium; Spinner flasks; Stationary phasis; Stirred tank bioreactors; Time points; Vaccine production; Vero; Vero cells; Wild types; Animals; Bioconversion; Bioreactors; Body fluids; Cell culture; Cells; Engineering research; Genes; Growth kinetics; Production engineering; Vaccines; Viruses; Diseases; cytodex 1; drug carrier; nonstructural protein 1; trypsin; unclassified drug; influenza vaccine; INS1 protein, influenza virus; live vaccine; virus protein; article; batch reactor; cell adhesion; cell culture; cell density; cell growth; cell viability; hemagglutination inhibition; influenza A; Influenza virus A; Influenza virus A H1N1; nonhuman; stirred reactor; Vero cell; virogenesis; virus replication; animal; bioreactor; biosynthesis; Cercopithecus; comparative study; culture medium; genetics; growth, development and aging; instrumentation; metabolism; methodology; phase contrast microscopy; physiology; virology; virus culture; Animalia; Orthomyxoviridae; Cercopithecus aethiops; Culture Media, Serum-Free; Influenza A Virus, H1N1 Subtype; Influenza Vaccines; Microscopy, Phase-Contrast; Vaccines, Attenuated; Vero Cells; Viral Nonstructural Proteins; Virus Cultivation; Virus Replication
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/181628
• Institution: National University of Singapore

10.1186/1472-6750-11-81