Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells

10.1186/1472-6750-11-81

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Main Authors:	Chen, A, Poh, S.L, Dietzsch, C, Roethl, E, Yan, M.L, Ng, S.K
Other Authors:	PHARMACY
Format:	Article
Published:	2020
Subjects:	Antiviral response Batch bioreactors Bioreactor system Cell concentrations Cell density Clinical trial Deletion mutants Efficient production Gene deletion Health concerns Human society Influenza Influenza vaccines Influenza virus Manufacturability Microcarrier Microcarrier culture Microcarriers Nonstructural proteins NS1 Phase I Production process Serum-free media Serum-free medium Spinner flasks Stationary phasis Stirred tank bioreactors Time points Vaccine production Vero Vero cells Wild types Animals Bioconversion Bioreactors Body fluids Cell culture Cells Engineering research Genes Growth kinetics Production engineering Vaccines Viruses Diseases cytodex 1 drug carrier nonstructural protein 1 trypsin unclassified drug influenza vaccine INS1 protein, influenza virus live vaccine virus protein article batch reactor cell adhesion cell culture cell density cell growth cell viability hemagglutination inhibition influenza A Influenza virus A Influenza virus A H1N1 nonhuman stirred reactor Vero cell virogenesis virus replication animal bioreactor biosynthesis Cercopithecus comparative study culture medium genetics growth, development and aging instrumentation metabolism methodology phase contrast microscopy physiology virology virus culture Animalia Orthomyxoviridae Cercopithecus aethiops Culture Media, Serum-Free Influenza A Virus, H1N1 Subtype Influenza Vaccines Microscopy, Phase-Contrast Vaccines, Attenuated Vero Cells Viral Nonstructural Proteins Virus Cultivation Virus Replication
Online Access:	https://scholarbank.nus.edu.sg/handle/10635/181628
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Institution:	National University of Singapore

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spelling	sg-nus-scholar.10635-1816282024-04-05T09:03:55Z Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells Chen, A Poh, S.L Dietzsch, C Roethl, E Yan, M.L Ng, S.K PHARMACY Antiviral response Batch bioreactors Bioreactor system Cell concentrations Cell density Clinical trial Deletion mutants Efficient production Gene deletion Health concerns Human society Influenza Influenza vaccines Influenza virus Manufacturability Microcarrier Microcarrier culture Microcarriers Nonstructural proteins NS1 Phase I Production process Serum-free media Serum-free medium Spinner flasks Stationary phasis Stirred tank bioreactors Time points Vaccine production Vero Vero cells Wild types Animals Bioconversion Bioreactors Body fluids Cell culture Cells Engineering research Genes Growth kinetics Production engineering Vaccines Viruses Diseases cytodex 1 drug carrier nonstructural protein 1 trypsin unclassified drug influenza vaccine INS1 protein, influenza virus live vaccine virus protein article batch reactor cell adhesion cell culture cell density cell growth cell viability hemagglutination inhibition influenza A Influenza virus A Influenza virus A H1N1 nonhuman stirred reactor Vero cell virogenesis virus replication animal bioreactor biosynthesis Cercopithecus comparative study culture medium genetics growth, development and aging Influenza virus A H1N1 instrumentation metabolism methodology phase contrast microscopy physiology Vero cell virology virus culture Animalia Orthomyxoviridae Animals Bioreactors Cercopithecus aethiops Culture Media, Serum-Free Influenza A Virus, H1N1 Subtype Influenza Vaccines Microscopy, Phase-Contrast Vaccines, Attenuated Vero Cells Viral Nonstructural Proteins Virus Cultivation Virus Replication 10.1186/1472-6750-11-81 BMC Biotechnology 11 81 2020-10-27T11:31:19Z 2020-10-27T11:31:19Z 2011 Article Chen, A, Poh, S.L, Dietzsch, C, Roethl, E, Yan, M.L, Ng, S.K (2011). Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells. BMC Biotechnology 11 : 81. ScholarBank@NUS Repository. https://doi.org/10.1186/1472-6750-11-81 14726750 https://scholarbank.nus.edu.sg/handle/10635/181628 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ Unpaywall 20201031
institution	National University of Singapore
building	NUS Library
continent	Asia
country	Singapore Singapore
content_provider	NUS Library
collection	ScholarBank@NUS
topic	Antiviral response Batch bioreactors Bioreactor system Cell concentrations Cell density Clinical trial Deletion mutants Efficient production Gene deletion Health concerns Human society Influenza Influenza vaccines Influenza virus Manufacturability Microcarrier Microcarrier culture Microcarriers Nonstructural proteins NS1 Phase I Production process Serum-free media Serum-free medium Spinner flasks Stationary phasis Stirred tank bioreactors Time points Vaccine production Vero Vero cells Wild types Animals Bioconversion Bioreactors Body fluids Cell culture Cells Engineering research Genes Growth kinetics Production engineering Vaccines Viruses Diseases cytodex 1 drug carrier nonstructural protein 1 trypsin unclassified drug influenza vaccine INS1 protein, influenza virus live vaccine virus protein article batch reactor cell adhesion cell culture cell density cell growth cell viability hemagglutination inhibition influenza A Influenza virus A Influenza virus A H1N1 nonhuman stirred reactor Vero cell virogenesis virus replication animal bioreactor biosynthesis Cercopithecus comparative study culture medium genetics growth, development and aging Influenza virus A H1N1 instrumentation metabolism methodology phase contrast microscopy physiology Vero cell virology virus culture Animalia Orthomyxoviridae Animals Bioreactors Cercopithecus aethiops Culture Media, Serum-Free Influenza A Virus, H1N1 Subtype Influenza Vaccines Microscopy, Phase-Contrast Vaccines, Attenuated Vero Cells Viral Nonstructural Proteins Virus Cultivation Virus Replication
spellingShingle	Antiviral response Batch bioreactors Bioreactor system Cell concentrations Cell density Clinical trial Deletion mutants Efficient production Gene deletion Health concerns Human society Influenza Influenza vaccines Influenza virus Manufacturability Microcarrier Microcarrier culture Microcarriers Nonstructural proteins NS1 Phase I Production process Serum-free media Serum-free medium Spinner flasks Stationary phasis Stirred tank bioreactors Time points Vaccine production Vero Vero cells Wild types Animals Bioconversion Bioreactors Body fluids Cell culture Cells Engineering research Genes Growth kinetics Production engineering Vaccines Viruses Diseases cytodex 1 drug carrier nonstructural protein 1 trypsin unclassified drug influenza vaccine INS1 protein, influenza virus live vaccine virus protein article batch reactor cell adhesion cell culture cell density cell growth cell viability hemagglutination inhibition influenza A Influenza virus A Influenza virus A H1N1 nonhuman stirred reactor Vero cell virogenesis virus replication animal bioreactor biosynthesis Cercopithecus comparative study culture medium genetics growth, development and aging Influenza virus A H1N1 instrumentation metabolism methodology phase contrast microscopy physiology Vero cell virology virus culture Animalia Orthomyxoviridae Animals Bioreactors Cercopithecus aethiops Culture Media, Serum-Free Influenza A Virus, H1N1 Subtype Influenza Vaccines Microscopy, Phase-Contrast Vaccines, Attenuated Vero Cells Viral Nonstructural Proteins Virus Cultivation Virus Replication Chen, A Poh, S.L Dietzsch, C Roethl, E Yan, M.L Ng, S.K Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells
description	10.1186/1472-6750-11-81
author2	PHARMACY
author_facet	PHARMACY Chen, A Poh, S.L Dietzsch, C Roethl, E Yan, M.L Ng, S.K
format	Article
author	Chen, A Poh, S.L Dietzsch, C Roethl, E Yan, M.L Ng, S.K
author_sort	Chen, A
title	Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells
title_short	Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells
title_full	Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells
title_fullStr	Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells
title_full_unstemmed	Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells
title_sort	serum-free microcarrier based production of replication deficient influenza vaccine candidate virus lacking ns1 using vero cells
publishDate	2020
url	https://scholarbank.nus.edu.sg/handle/10635/181628
_version_	1800914646841950208

Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells

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