Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9

STAR PROTOCOLS

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Main Authors: Chan, Dedrick Kok Hong, Collins, Scott David, Buczacki, Simon James Alexander
Other Authors: SURGERY
Format: Article
Language:English
Published: ELSEVIER 2023
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Online Access:https://scholarbank.nus.edu.sg/handle/10635/245646
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Institution: National University of Singapore
Language: English
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spelling sg-nus-scholar.10635-2456462024-04-24T08:41:27Z Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9 Chan, Dedrick Kok Hong Collins, Scott David Buczacki, Simon James Alexander SURGERY Science & Technology Life Sciences & Biomedicine Biochemical Research Methods Biochemistry & Molecular Biology STAR PROTOCOLS 4 1 2023-10-31T04:29:00Z 2023-10-31T04:29:00Z 2023-03-17 2023-10-30T06:38:42Z Article Chan, Dedrick Kok Hong, Collins, Scott David, Buczacki, Simon James Alexander (2023-03-17). Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9. STAR PROTOCOLS 4 (1). ScholarBank@NUS Repository. 2666-1667 https://scholarbank.nus.edu.sg/handle/10635/245646 en ELSEVIER Elements
institution National University of Singapore
building NUS Library
continent Asia
country Singapore
Singapore
content_provider NUS Library
collection ScholarBank@NUS
language English
topic Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
spellingShingle Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
Chan, Dedrick Kok Hong
Collins, Scott David
Buczacki, Simon James Alexander
Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9
description STAR PROTOCOLS
author2 SURGERY
author_facet SURGERY
Chan, Dedrick Kok Hong
Collins, Scott David
Buczacki, Simon James Alexander
format Article
author Chan, Dedrick Kok Hong
Collins, Scott David
Buczacki, Simon James Alexander
author_sort Chan, Dedrick Kok Hong
title Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9
title_short Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9
title_full Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9
title_fullStr Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9
title_full_unstemmed Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9
title_sort generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide rna crispr-cas9
publisher ELSEVIER
publishDate 2023
url https://scholarbank.nus.edu.sg/handle/10635/245646
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