Prognostic impact of P53 status, TLS-CHOP fusion transcript structure, and histological grade in myxoid liposarcoma: A molecular and clinicopathologic study of 82 cases
Purpose: A specific TLS-CHOP fusion gene resulting from the t(12;16) is present in at least 95% of myxoid liposarcomas (MLS). Three common forms of the TLS-CHOP fusion have been described, differing by the presence or absence of TLS exons 6-8 in the fusion product. Type 5-2 (also known as type II) c...
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2001
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sg-smu-ink.soe_research-34872021-09-08T07:59:20Z Prognostic impact of P53 status, TLS-CHOP fusion transcript structure, and histological grade in myxoid liposarcoma: A molecular and clinicopathologic study of 82 cases Antonescu, C. R. Tschernyavsky, S. J. Decuseara, R. LEUNG, Denis H. Y. Woodruff, J. M. Brennan, M. F. Bridge, J. A. Neff, J. R. Goldblum, J. R. Ladanyi, M. Purpose: A specific TLS-CHOP fusion gene resulting from the t(12;16) is present in at least 95% of myxoid liposarcomas (MLS). Three common forms of the TLS-CHOP fusion have been described, differing by the presence or absence of TLS exons 6-8 in the fusion product. Type 5-2 (also known as type II) consists of TLS exons 1-5 fused to CHOP exon 2; type 7-2 (also known as type I) also includes TLS exons 6 and 7 in the fusion, whereas type 8-2 (also known as type III) fuses TLS exons 1-8 to CHOP exon 2. We sought to determine the impact of TLS-CHOP fusion transcript structure on clinical outcome in a group of well-characterized MLS cases. We also analyzed P53 status, because this parameter has been found to have a significant prognostic impact in other sarcomas with chromosomal translocations. Methods: We analyzed TLS-CHOP fusion transcripts by reverse-transcription PCR using RNA extracted from frozen tissue in 82 MLS confirmed previously to harbor a CHOP rearrangement either by Southern blotting or by cytogenetic detection of the t(12;16). Parameters analyzed included age, location, size, percentage of round cell (RC) component, areas of increased cellularity, necrosis, and surgical margins. In 71 (87%) cases, adequate tumor tissue was available for immunohistochemical analysis of P53 status, using DO7 antibody. The Kaplan-Meier method, log-rank, and Cox regression tests were used for survival analyses. Results: Most MLS were >10 cm (73%), arising in the thigh (70%), and localized at presentation (89%). RC component was 2001-12-01T08:00:00Z text application/pdf https://ink.library.smu.edu.sg/soe_research/2488 https://ink.library.smu.edu.sg/context/soe_research/article/3487/viewcontent/P53_CCR_3977.full_2001_pv.pdf http://creativecommons.org/licenses/by-nc-nd/4.0/ Research Collection School Of Economics eng Institutional Knowledge at Singapore Management University protein p53 cancer Econometrics Medicine and Health Sciences |
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protein p53 cancer Econometrics Medicine and Health Sciences Antonescu, C. R. Tschernyavsky, S. J. Decuseara, R. LEUNG, Denis H. Y. Woodruff, J. M. Brennan, M. F. Bridge, J. A. Neff, J. R. Goldblum, J. R. Ladanyi, M. Prognostic impact of P53 status, TLS-CHOP fusion transcript structure, and histological grade in myxoid liposarcoma: A molecular and clinicopathologic study of 82 cases |
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Purpose: A specific TLS-CHOP fusion gene resulting from the t(12;16) is present in at least 95% of myxoid liposarcomas (MLS). Three common forms of the TLS-CHOP fusion have been described, differing by the presence or absence of TLS exons 6-8 in the fusion product. Type 5-2 (also known as type II) consists of TLS exons 1-5 fused to CHOP exon 2; type 7-2 (also known as type I) also includes TLS exons 6 and 7 in the fusion, whereas type 8-2 (also known as type III) fuses TLS exons 1-8 to CHOP exon 2. We sought to determine the impact of TLS-CHOP fusion transcript structure on clinical outcome in a group of well-characterized MLS cases. We also analyzed P53 status, because this parameter has been found to have a significant prognostic impact in other sarcomas with chromosomal translocations. Methods: We analyzed TLS-CHOP fusion transcripts by reverse-transcription PCR using RNA extracted from frozen tissue in 82 MLS confirmed previously to harbor a CHOP rearrangement either by Southern blotting or by cytogenetic detection of the t(12;16). Parameters analyzed included age, location, size, percentage of round cell (RC) component, areas of increased cellularity, necrosis, and surgical margins. In 71 (87%) cases, adequate tumor tissue was available for immunohistochemical analysis of P53 status, using DO7 antibody. The Kaplan-Meier method, log-rank, and Cox regression tests were used for survival analyses. Results: Most MLS were >10 cm (73%), arising in the thigh (70%), and localized at presentation (89%). RC component was |
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Antonescu, C. R. Tschernyavsky, S. J. Decuseara, R. LEUNG, Denis H. Y. Woodruff, J. M. Brennan, M. F. Bridge, J. A. Neff, J. R. Goldblum, J. R. Ladanyi, M. |
author_facet |
Antonescu, C. R. Tschernyavsky, S. J. Decuseara, R. LEUNG, Denis H. Y. Woodruff, J. M. Brennan, M. F. Bridge, J. A. Neff, J. R. Goldblum, J. R. Ladanyi, M. |
author_sort |
Antonescu, C. R. |
title |
Prognostic impact of P53 status, TLS-CHOP fusion transcript structure, and histological grade in myxoid liposarcoma: A molecular and clinicopathologic study of 82 cases |
title_short |
Prognostic impact of P53 status, TLS-CHOP fusion transcript structure, and histological grade in myxoid liposarcoma: A molecular and clinicopathologic study of 82 cases |
title_full |
Prognostic impact of P53 status, TLS-CHOP fusion transcript structure, and histological grade in myxoid liposarcoma: A molecular and clinicopathologic study of 82 cases |
title_fullStr |
Prognostic impact of P53 status, TLS-CHOP fusion transcript structure, and histological grade in myxoid liposarcoma: A molecular and clinicopathologic study of 82 cases |
title_full_unstemmed |
Prognostic impact of P53 status, TLS-CHOP fusion transcript structure, and histological grade in myxoid liposarcoma: A molecular and clinicopathologic study of 82 cases |
title_sort |
prognostic impact of p53 status, tls-chop fusion transcript structure, and histological grade in myxoid liposarcoma: a molecular and clinicopathologic study of 82 cases |
publisher |
Institutional Knowledge at Singapore Management University |
publishDate |
2001 |
url |
https://ink.library.smu.edu.sg/soe_research/2488 https://ink.library.smu.edu.sg/context/soe_research/article/3487/viewcontent/P53_CCR_3977.full_2001_pv.pdf |
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1770575826753945600 |