Effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture

Osteoarthritis (OA) is a degenerative joint disease that progressively causes a loss of joint functions and the impaired quality of life. The most significant event in OA is a high degree of degradation of articular cartilage accompanied by the loss of chondroitin sulfate-proteoglycans (CS-PGs). Rec...

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Main Authors: Pothacharoen P., Najarus S., Settakorn J., Mizumoto S., Sugahara K., Kongtawelert P.
Format: Article
Language:English
Published: Kluwer Academic Publishers 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84897495433&partnerID=40&md5=2a0bfb7c256d6323daec27ecf0347ac9
http://cmuir.cmu.ac.th/handle/6653943832/1705
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spelling th-cmuir.6653943832-17052014-08-30T01:59:57Z Effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture Pothacharoen P. Najarus S. Settakorn J. Mizumoto S. Sugahara K. Kongtawelert P. Osteoarthritis (OA) is a degenerative joint disease that progressively causes a loss of joint functions and the impaired quality of life. The most significant event in OA is a high degree of degradation of articular cartilage accompanied by the loss of chondroitin sulfate-proteoglycans (CS-PGs). Recently, the chondroprotective effects of sesamin, the naturally occurring substance found in sesame seeds, have been proved in a rat model of papain-induced osteoarthritis. We hypothesized that sesamin may be associated with possible promotion of the biosynthesis of CS-PGs in human articular chondrocytes. The aim of the study was to investigate the effects of sesamin on the major CS-PG biosynthesis in primary human chondrocyte. The effects of sesamin on the gene expression of the PG core and the CS biosynthetic enzymes as well as on the secretion of glycosaminoglycans (GAGs) in monolayer and pellet culture systems of articular chondrocytes. Sesamin significantly increased the GAGs content both in culture medium and pellet matrix. Real-time-quantitative PCR showed that sesamin promoted the expression of the genes encoding the core protein (ACAN) of the major CS-PG aggrecan and the biosynthetic enzymes (XYLT1, XYLT2, CHSY1 and CHPF) required for the synthesis of CS-GAG side chains. Safranin-O staining of sesamin treated chondrocyte pellet section confirmed the high degree of GAG accumulation. These results were correlated with an increased level of secreted GAGs in the media of cultured articular chondrocytes in both culture systems. Thus, sesamin would provide a potential therapeutic strategy for treating OA patients. © 2013 Springer Science+Business Media. 2014-08-30T01:59:57Z 2014-08-30T01:59:57Z 2014 Article 15734986 10.1007/s10719-013-9514-6 GLJOE http://www.scopus.com/inward/record.url?eid=2-s2.0-84897495433&partnerID=40&md5=2a0bfb7c256d6323daec27ecf0347ac9 http://cmuir.cmu.ac.th/handle/6653943832/1705 English Kluwer Academic Publishers
institution Chiang Mai University
building Chiang Mai University Library
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language English
description Osteoarthritis (OA) is a degenerative joint disease that progressively causes a loss of joint functions and the impaired quality of life. The most significant event in OA is a high degree of degradation of articular cartilage accompanied by the loss of chondroitin sulfate-proteoglycans (CS-PGs). Recently, the chondroprotective effects of sesamin, the naturally occurring substance found in sesame seeds, have been proved in a rat model of papain-induced osteoarthritis. We hypothesized that sesamin may be associated with possible promotion of the biosynthesis of CS-PGs in human articular chondrocytes. The aim of the study was to investigate the effects of sesamin on the major CS-PG biosynthesis in primary human chondrocyte. The effects of sesamin on the gene expression of the PG core and the CS biosynthetic enzymes as well as on the secretion of glycosaminoglycans (GAGs) in monolayer and pellet culture systems of articular chondrocytes. Sesamin significantly increased the GAGs content both in culture medium and pellet matrix. Real-time-quantitative PCR showed that sesamin promoted the expression of the genes encoding the core protein (ACAN) of the major CS-PG aggrecan and the biosynthetic enzymes (XYLT1, XYLT2, CHSY1 and CHPF) required for the synthesis of CS-GAG side chains. Safranin-O staining of sesamin treated chondrocyte pellet section confirmed the high degree of GAG accumulation. These results were correlated with an increased level of secreted GAGs in the media of cultured articular chondrocytes in both culture systems. Thus, sesamin would provide a potential therapeutic strategy for treating OA patients. © 2013 Springer Science+Business Media.
format Article
author Pothacharoen P.
Najarus S.
Settakorn J.
Mizumoto S.
Sugahara K.
Kongtawelert P.
spellingShingle Pothacharoen P.
Najarus S.
Settakorn J.
Mizumoto S.
Sugahara K.
Kongtawelert P.
Effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture
author_facet Pothacharoen P.
Najarus S.
Settakorn J.
Mizumoto S.
Sugahara K.
Kongtawelert P.
author_sort Pothacharoen P.
title Effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture
title_short Effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture
title_full Effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture
title_fullStr Effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture
title_full_unstemmed Effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture
title_sort effects of sesamin on the biosynthesis of chondroitin sulfate proteoglycans in human articular chondrocytes in primary culture
publisher Kluwer Academic Publishers
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84897495433&partnerID=40&md5=2a0bfb7c256d6323daec27ecf0347ac9
http://cmuir.cmu.ac.th/handle/6653943832/1705
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