Structural characterization of human liver heparan sulfate

• Main Authors: Vongchan P., Warda M., Toyoda H., Toida T., Marks R.M., Linhardt R.J.
• 格式: Article
• 語言: English
• 出版: 2014
• 在線閱讀: http://www.scopus.com/inward/record.url?eid=2-s2.0-11844304289&partnerID=40&md5=1c0fccd75003a66bba1c7d080022a882; http://www.ncbi.nlm.nih.gov/pubmed/15652173; http://cmuir.cmu.ac.th/handle/6653943832/1963

The isolation, purification and structural characterization of human liver heparan sulfate are described. 1H-NMR spectroscopy demonstrates the purity of this glycosaminoglycan (GAG) and two-dimensional 1H-NMR confirmed that it was heparan sulfate. Enzymatic depolymerization of the isolated heparan s...