Construction of infectious dengue 2 virus cDNA clones using high copy number plasmid

• Main Authors: Sriburi R., Keelapang P., Duangchinda T., Pruksakorn S., Maneekarn N., Malasit P., Sittisombut N.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-0035153235&partnerID=40&md5=6a5aa6a4303470460669656e32e8f386; http://www.ncbi.nlm.nih.gov/pubmed/11164920; http://cmuir.cmu.ac.th/handle/6653943832/1997
• Institution: Chiang Mai University

Procedures for cloning entire dengue serotype 2 virus genome in the multiple cloning site of a commercially available high copy number plasmid are described. The 10.7 kb viral RNA genome was reverse transcribed, amplified as three overlapping DNA fragments and successively ligated into pBluescript I...