Construction of infectious dengue 2 virus cDNA clones using high copy number plasmid
Procedures for cloning entire dengue serotype 2 virus genome in the multiple cloning site of a commercially available high copy number plasmid are described. The 10.7 kb viral RNA genome was reverse transcribed, amplified as three overlapping DNA fragments and successively ligated into pBluescript I...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2014
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| Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0035153235&partnerID=40&md5=6a5aa6a4303470460669656e32e8f386 http://www.ncbi.nlm.nih.gov/pubmed/11164920 http://cmuir.cmu.ac.th/handle/6653943832/1997 |
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| Institution: | Chiang Mai University |
| Language: | English |