Mass spectrometry-based detection of hemoglobin E mutation by allele-specific base extension reaction

• Main Authors: Tsang J.C.H., Charoenkwan P., Chow K.C.K., Jin Y., Wanapirak C., Sanguansermsri T., Lo Y.M.D., Chiu R.W.K.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-36649013864&partnerID=40&md5=c56e771376d52fe8ef702dcb54037ee0; http://cmuir.cmu.ac.th/handle/6653943832/2093
• Institution: Chiang Mai University

Background: The specific detection of a minor population of mutant DNA molecules requires methods of high specificity and sensitivity. While the single-allele base extension reaction (SABER) was shown to be useful for the detection of certain beta-thalassemia mutations, we encountered problems with...