Rapid detection of Brugia malayi in mosquito vectors using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
We developed real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) combined with melting curve analysis for detection of Brugia malayi DNA in blood-fed mosquitoes. Real-time FRET PCR is based on a fluorescence melting curve analysis of hybrid formed between amplicon...
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Main Authors: | Thanchomnang T., Intapan P.M., Lulitanond V., Choochote W., Manjai A., Prasongdee T.K., Maleewong W. |
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Format: | Article |
Language: | English |
Published: |
2014
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Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-42949097929&partnerID=40&md5=396fa0e561ebb43e62b2e07bafaebee9 http://cmuir.cmu.ac.th/handle/6653943832/2471 |
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Institution: | Chiang Mai University |
Language: | English |
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