Rapid detection of Wuchereria bancrofti in mosquitoes by LightCycler polymerase chain reaction and melting curve analysis

• المؤلفون الرئيسيون: Lulitanond V., Intapan P.M., Pipitgool V., Choochote W., Maleewong W.
• التنسيق: مقال
• اللغة: English
• منشور في: 2014
• الوصول للمادة أونلاين: http://www.scopus.com/inward/record.url?eid=2-s2.0-11144291086&partnerID=40&md5=f5ab4d548895806794229d1e6c4110dd; http://cmuir.cmu.ac.th/handle/6653943832/3774
• المؤسسة: Chiang Mai University

A LightCycler real-time polymerase chain reaction (PCR) assay was developed to detect Wuchereria bancrofti DNA in blood-fed mosquitoes. The assay is based on fluorescence melting curve analysis of the PCR product generated from a family of repeated DNA elements: the 182 bp SspI repeat, specific to t...