Rapid detection of Wuchereria bancrofti in mosquitoes by LightCycler polymerase chain reaction and melting curve analysis

• Main Authors: Lulitanond V., Intapan P.M., Pipitgool V., Choochote W., Maleewong W.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-11144291086&partnerID=40&md5=f5ab4d548895806794229d1e6c4110dd; http://cmuir.cmu.ac.th/handle/6653943832/3774

A LightCycler real-time polymerase chain reaction (PCR) assay was developed to detect Wuchereria bancrofti DNA in blood-fed mosquitoes. The assay is based on fluorescence melting curve analysis of the PCR product generated from a family of repeated DNA elements: the 182 bp SspI repeat, specific to t...