Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2014
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| Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-70149093169&partnerID=40&md5=8308198335a7a1a3bfaacb9f78ba59a5 http://www.ncbi.nlm.nih.gov/pubmed/19722076 http://cmuir.cmu.ac.th/handle/6653943832/2833 |
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| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggest-ing a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies varia-tion (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms. |
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