Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand

Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of...

Full description

Saved in:
Bibliographic Details
Main Authors: Thongsahuan S., Baimai V., Otsuka Y., Saeung A., Tuetun B., Jariyapan N., Suwannamit S., Somboon P., Jitpakdi A., Takaoka H., Choochote W.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-70149093169&partnerID=40&md5=8308198335a7a1a3bfaacb9f78ba59a5
http://www.ncbi.nlm.nih.gov/pubmed/19722076
http://cmuir.cmu.ac.th/handle/6653943832/2833
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Language: English
id th-cmuir.6653943832-2833
record_format dspace
spelling th-cmuir.6653943832-28332014-08-30T02:25:26Z Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand Thongsahuan S. Baimai V. Otsuka Y. Saeung A. Tuetun B. Jariyapan N. Suwannamit S. Somboon P. Jitpakdi A. Takaoka H. Choochote W. Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggest-ing a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies varia-tion (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms. 2014-08-30T02:25:26Z 2014-08-30T02:25:26Z 2009 Article 00740276 19722076 http://www.scopus.com/inward/record.url?eid=2-s2.0-70149093169&partnerID=40&md5=8308198335a7a1a3bfaacb9f78ba59a5 http://www.ncbi.nlm.nih.gov/pubmed/19722076 http://cmuir.cmu.ac.th/handle/6653943832/2833 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggest-ing a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies varia-tion (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms.
format Article
author Thongsahuan S.
Baimai V.
Otsuka Y.
Saeung A.
Tuetun B.
Jariyapan N.
Suwannamit S.
Somboon P.
Jitpakdi A.
Takaoka H.
Choochote W.
spellingShingle Thongsahuan S.
Baimai V.
Otsuka Y.
Saeung A.
Tuetun B.
Jariyapan N.
Suwannamit S.
Somboon P.
Jitpakdi A.
Takaoka H.
Choochote W.
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
author_facet Thongsahuan S.
Baimai V.
Otsuka Y.
Saeung A.
Tuetun B.
Jariyapan N.
Suwannamit S.
Somboon P.
Jitpakdi A.
Takaoka H.
Choochote W.
author_sort Thongsahuan S.
title Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
title_short Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
title_full Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
title_fullStr Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
title_full_unstemmed Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
title_sort karyotypic variation and geographic distribution of anopheles campestris-like (diptera: culicidae) in thailand
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-70149093169&partnerID=40&md5=8308198335a7a1a3bfaacb9f78ba59a5
http://www.ncbi.nlm.nih.gov/pubmed/19722076
http://cmuir.cmu.ac.th/handle/6653943832/2833
_version_ 1681419933852893184