Broad usage spectrum of G protein-coupled receptors as coreceptors by primary isolates of HIV

Objective: HIV-1 can use various G protein-coupled receptors (GPCRs) in addition to CCR5 and CXCR4 as coreceptors;however, this type of HIV-1 infection has hardly been detected in vivo. The objective of this study was to elucidate the spectrum of GPCR usage by HIV-1 populations in vivo. Design: CD4-...

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Main Authors: Shimizu N., Tanaka A., Oue A., Mori T., Ohtsuki T., Apichartpiyakul C., Uchiumi H., Nojima Y., Hoshino H.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-67649668892&partnerID=40&md5=19fac9e0c30536bd84339e916862c977
http://cmuir.cmu.ac.th/handle/6653943832/2869
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spelling th-cmuir.6653943832-28692014-08-30T02:25:29Z Broad usage spectrum of G protein-coupled receptors as coreceptors by primary isolates of HIV Shimizu N. Tanaka A. Oue A. Mori T. Ohtsuki T. Apichartpiyakul C. Uchiumi H. Nojima Y. Hoshino H. Objective: HIV-1 can use various G protein-coupled receptors (GPCRs) in addition to CCR5 and CXCR4 as coreceptors;however, this type of HIV-1 infection has hardly been detected in vivo. The objective of this study was to elucidate the spectrum of GPCR usage by HIV-1 populations in vivo. Design: CD4-expressing glioma cell line, NP-2/CD4, becomes highly susceptible to HIV-1 when the cells express GPCRs with coreceptor activities. This cell system was advantageous for detecting the inefficient use of GPCRs by HIV-1. Methods: We developed NP-2/CD4/GPCR cells that express each of 23 GPCRs: 21 chemokine receptors (CCR1, CCR2b, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9B, CCR10, CCR11, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, CX3CR1, XCR1, D6, and DARC) and two other GPCRs (a formylpeptide receptor, FPRL1, and an orphan GPCR, GPR1). NP-2/CD4/GPCR cells were directly cocultured with HIV-1-positive peripheral blood lymphocytes and HIV-1 infection was detected. Results: Primary HIV-1 isolates were obtained from NP-2/CD4/GPCR cells expressing CCR5, CXCR4, FPRL1, or GPR1 cocultured with 11 of 17 peripheral blood lymphocytes. Surprisingly, these isolates showed extremely expanded GPCR usage, such as CCR1, CCR3, CCR5, CCR8, CXCR4, D6, FPRL1, and GPR1 as coreceptors. We found that CCR9B, CCR10, and XCR1 also work as novel HIV-1 coreceptors. Conclusion: FPRL1 and GPR1 have the potential to work as significant HIV-1 cor- eceptors in vivo next to CCR5 and CXCR4. HIV-1 populations that can use various GPCRs as coreceptors are already circulating in vivo, even in the early stage of HIV-1 infection. © 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins. 2014-08-30T02:25:29Z 2014-08-30T02:25:29Z 2009 Article 02699370 10.1097/QAD.0b013e328326cc0d 19307942 AIDSE http://www.scopus.com/inward/record.url?eid=2-s2.0-67649668892&partnerID=40&md5=19fac9e0c30536bd84339e916862c977 http://cmuir.cmu.ac.th/handle/6653943832/2869 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Objective: HIV-1 can use various G protein-coupled receptors (GPCRs) in addition to CCR5 and CXCR4 as coreceptors;however, this type of HIV-1 infection has hardly been detected in vivo. The objective of this study was to elucidate the spectrum of GPCR usage by HIV-1 populations in vivo. Design: CD4-expressing glioma cell line, NP-2/CD4, becomes highly susceptible to HIV-1 when the cells express GPCRs with coreceptor activities. This cell system was advantageous for detecting the inefficient use of GPCRs by HIV-1. Methods: We developed NP-2/CD4/GPCR cells that express each of 23 GPCRs: 21 chemokine receptors (CCR1, CCR2b, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9B, CCR10, CCR11, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, CX3CR1, XCR1, D6, and DARC) and two other GPCRs (a formylpeptide receptor, FPRL1, and an orphan GPCR, GPR1). NP-2/CD4/GPCR cells were directly cocultured with HIV-1-positive peripheral blood lymphocytes and HIV-1 infection was detected. Results: Primary HIV-1 isolates were obtained from NP-2/CD4/GPCR cells expressing CCR5, CXCR4, FPRL1, or GPR1 cocultured with 11 of 17 peripheral blood lymphocytes. Surprisingly, these isolates showed extremely expanded GPCR usage, such as CCR1, CCR3, CCR5, CCR8, CXCR4, D6, FPRL1, and GPR1 as coreceptors. We found that CCR9B, CCR10, and XCR1 also work as novel HIV-1 coreceptors. Conclusion: FPRL1 and GPR1 have the potential to work as significant HIV-1 cor- eceptors in vivo next to CCR5 and CXCR4. HIV-1 populations that can use various GPCRs as coreceptors are already circulating in vivo, even in the early stage of HIV-1 infection. © 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins.
format Article
author Shimizu N.
Tanaka A.
Oue A.
Mori T.
Ohtsuki T.
Apichartpiyakul C.
Uchiumi H.
Nojima Y.
Hoshino H.
spellingShingle Shimizu N.
Tanaka A.
Oue A.
Mori T.
Ohtsuki T.
Apichartpiyakul C.
Uchiumi H.
Nojima Y.
Hoshino H.
Broad usage spectrum of G protein-coupled receptors as coreceptors by primary isolates of HIV
author_facet Shimizu N.
Tanaka A.
Oue A.
Mori T.
Ohtsuki T.
Apichartpiyakul C.
Uchiumi H.
Nojima Y.
Hoshino H.
author_sort Shimizu N.
title Broad usage spectrum of G protein-coupled receptors as coreceptors by primary isolates of HIV
title_short Broad usage spectrum of G protein-coupled receptors as coreceptors by primary isolates of HIV
title_full Broad usage spectrum of G protein-coupled receptors as coreceptors by primary isolates of HIV
title_fullStr Broad usage spectrum of G protein-coupled receptors as coreceptors by primary isolates of HIV
title_full_unstemmed Broad usage spectrum of G protein-coupled receptors as coreceptors by primary isolates of HIV
title_sort broad usage spectrum of g protein-coupled receptors as coreceptors by primary isolates of hiv
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-67649668892&partnerID=40&md5=19fac9e0c30536bd84339e916862c977
http://cmuir.cmu.ac.th/handle/6653943832/2869
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