Current molecular epidemiology and recombination of HIV type 1 subtypes in Northern Thailand

HIV subtype characterization is an important tool to monitor the genetic variation of the HIV epidemic. This study investigated the current HIV subtype distribution and recombination among the northern Thai population. An in-house genotypic assay of HIV protease and reverse transcriptase genes was p...

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Main Authors: Praparattanapan J., Tragoolpua Y., Pathom-Aree W., Kotarathitithum W., Chaiwarith R., Nuntachit N., Sirisanthana T., Supparatpinyo K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-80655125221&partnerID=40&md5=ec15829eeda4fbe122ac1310a026c195
http://www.ncbi.nlm.nih.gov/pubmed/21449850
http://cmuir.cmu.ac.th/handle/6653943832/2979
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-29792014-08-30T02:25:37Z Current molecular epidemiology and recombination of HIV type 1 subtypes in Northern Thailand Praparattanapan J. Tragoolpua Y. Pathom-Aree W. Kotarathitithum W. Chaiwarith R. Nuntachit N. Sirisanthana T. Supparatpinyo K. HIV subtype characterization is an important tool to monitor the genetic variation of the HIV epidemic. This study investigated the current HIV subtype distribution and recombination among the northern Thai population. An in-house genotypic assay of HIV protease and reverse transcriptase genes was performed on 420 plasma specimens from HIV-infected patients residing in several northern Thai provinces. HIV subtyping was determined by phylogenetic analysis. Three hundred and ninety-eight sequences (94.8%) were identified as CRF01-AE with the genetic distance of 1.848±0.957% and 12 (2.9%) as subtype B with the genetic distance of 4.186±0.849%. In addition, two sequences (0.5%) of HIV subtype C were found, suggesting that these patients were either immigrants from another country or were infected through heterosexual contact with HIV-infected subjects from another country. Bootscan analysis showed that there were eight (1.9%) unique recombinant forms (URFs) consisting of a recombinant of CRF01-AE with subtype B or subtype C. The information from this study is useful for prevention programs to halt the onward transmission of a particular HIV outbreak. However, characterization of the full genome of these CRF01-AE/B and CRF01-AE/C intersubtype recombinants, and also subtype C, is required for confirmation and elucidation. © 2011, Mary Ann Liebert, Inc. 2014-08-30T02:25:37Z 2014-08-30T02:25:37Z 2011 Article 8892229 10.1089/aid.2010.0257 ARHRE http://www.scopus.com/inward/record.url?eid=2-s2.0-80655125221&partnerID=40&md5=ec15829eeda4fbe122ac1310a026c195 http://www.ncbi.nlm.nih.gov/pubmed/21449850 http://cmuir.cmu.ac.th/handle/6653943832/2979 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description HIV subtype characterization is an important tool to monitor the genetic variation of the HIV epidemic. This study investigated the current HIV subtype distribution and recombination among the northern Thai population. An in-house genotypic assay of HIV protease and reverse transcriptase genes was performed on 420 plasma specimens from HIV-infected patients residing in several northern Thai provinces. HIV subtyping was determined by phylogenetic analysis. Three hundred and ninety-eight sequences (94.8%) were identified as CRF01-AE with the genetic distance of 1.848±0.957% and 12 (2.9%) as subtype B with the genetic distance of 4.186±0.849%. In addition, two sequences (0.5%) of HIV subtype C were found, suggesting that these patients were either immigrants from another country or were infected through heterosexual contact with HIV-infected subjects from another country. Bootscan analysis showed that there were eight (1.9%) unique recombinant forms (URFs) consisting of a recombinant of CRF01-AE with subtype B or subtype C. The information from this study is useful for prevention programs to halt the onward transmission of a particular HIV outbreak. However, characterization of the full genome of these CRF01-AE/B and CRF01-AE/C intersubtype recombinants, and also subtype C, is required for confirmation and elucidation. © 2011, Mary Ann Liebert, Inc.
format Article
author Praparattanapan J.
Tragoolpua Y.
Pathom-Aree W.
Kotarathitithum W.
Chaiwarith R.
Nuntachit N.
Sirisanthana T.
Supparatpinyo K.
spellingShingle Praparattanapan J.
Tragoolpua Y.
Pathom-Aree W.
Kotarathitithum W.
Chaiwarith R.
Nuntachit N.
Sirisanthana T.
Supparatpinyo K.
Current molecular epidemiology and recombination of HIV type 1 subtypes in Northern Thailand
author_facet Praparattanapan J.
Tragoolpua Y.
Pathom-Aree W.
Kotarathitithum W.
Chaiwarith R.
Nuntachit N.
Sirisanthana T.
Supparatpinyo K.
author_sort Praparattanapan J.
title Current molecular epidemiology and recombination of HIV type 1 subtypes in Northern Thailand
title_short Current molecular epidemiology and recombination of HIV type 1 subtypes in Northern Thailand
title_full Current molecular epidemiology and recombination of HIV type 1 subtypes in Northern Thailand
title_fullStr Current molecular epidemiology and recombination of HIV type 1 subtypes in Northern Thailand
title_full_unstemmed Current molecular epidemiology and recombination of HIV type 1 subtypes in Northern Thailand
title_sort current molecular epidemiology and recombination of hiv type 1 subtypes in northern thailand
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-80655125221&partnerID=40&md5=ec15829eeda4fbe122ac1310a026c195
http://www.ncbi.nlm.nih.gov/pubmed/21449850
http://cmuir.cmu.ac.th/handle/6653943832/2979
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