Detailed investigation of factors influencing amplification efficiency and allele drop-out in single cell PCR: Implications for preimplantation genetic diagnosis

• Main Authors: Piyamongkol W., Bermudez M.G., Harper J.C., Wells D.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-0038148733&partnerID=40&md5=254d2a986b0e4acfafb9992324491855; http://cmuir.cmu.ac.th/handle/6653943832/2987
• Institution: Chiang Mai University

Preimplantation genetic diagnosis (PGD) of single gene disorders relies on PCR-based tests performed on single cells (polar bodies or blastomeres). Despite the use of increasingly robust protocols, allele drop-out (ADO; the failure to amplify one of the two alleles in a heterozygous cell) remains a...