Relative contribution of OAT1 and OAT3 transport activities in isolated perfused rabbit renal proximal tubules

The expression of both OAT1 and OAT3 along the isolated rabbit renal proximal tubule (RPT) was determined using RT-PCR. They were found to be very strong in S2 segment and weak in S1 and S3 segments. We further examined the relative transport activity of these transporters in isolated perfused rabbi...

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Main Authors: Lungkaphin A., Lewchalermwongse B., Chatsudthipong V.
Format: In Vitro
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/3179
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-31792014-08-30T02:25:51Z Relative contribution of OAT1 and OAT3 transport activities in isolated perfused rabbit renal proximal tubules Lungkaphin A. Lewchalermwongse B. Chatsudthipong V. The expression of both OAT1 and OAT3 along the isolated rabbit renal proximal tubule (RPT) was determined using RT-PCR. They were found to be very strong in S2 segment and weak in S1 and S3 segments. We further examined the relative transport activity of these transporters in isolated perfused rabbit RPT using [(3)H]para-aminohippurate ([(3)H]PAH), and estrone sulfate ([(3)H]ES) as specific substrates for rbOAT1 and rbOAT3, respectively. The transport activity of OAT1 was in the order S2>S1=S3 segments and that of OAT3 was in the order S1=S2>S3 segments. The addition of alpha-ketoglutarate (100 muM) in the bathing medium increased both OAT1 and OAT3 transport activities in all segments of proximal tubule. The kinetics of [(3)H]succinic acid transport, used to measure the activity of sodium dicarboxylate transporter 3 (NaDC3), were examined. The J(max) for succinic acid was in the order S2>S3 and unmeasurable in the S1 segment. Our data indicate that both OAT1 and OAT3 play quantitatively significant roles in the renal transport of organic anions along the proximal tubule but predominately in S2 segment. The relative contribution of both transporters depends on their relative expression levels and may possibly be affected by the activity of NaDC3 in RPT. 2014-08-30T02:25:51Z 2014-08-30T02:25:51Z 2006 In Vitro 0006-3002 16815243 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/3179 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The expression of both OAT1 and OAT3 along the isolated rabbit renal proximal tubule (RPT) was determined using RT-PCR. They were found to be very strong in S2 segment and weak in S1 and S3 segments. We further examined the relative transport activity of these transporters in isolated perfused rabbit RPT using [(3)H]para-aminohippurate ([(3)H]PAH), and estrone sulfate ([(3)H]ES) as specific substrates for rbOAT1 and rbOAT3, respectively. The transport activity of OAT1 was in the order S2>S1=S3 segments and that of OAT3 was in the order S1=S2>S3 segments. The addition of alpha-ketoglutarate (100 muM) in the bathing medium increased both OAT1 and OAT3 transport activities in all segments of proximal tubule. The kinetics of [(3)H]succinic acid transport, used to measure the activity of sodium dicarboxylate transporter 3 (NaDC3), were examined. The J(max) for succinic acid was in the order S2>S3 and unmeasurable in the S1 segment. Our data indicate that both OAT1 and OAT3 play quantitatively significant roles in the renal transport of organic anions along the proximal tubule but predominately in S2 segment. The relative contribution of both transporters depends on their relative expression levels and may possibly be affected by the activity of NaDC3 in RPT.
format In Vitro
author Lungkaphin A.
Lewchalermwongse B.
Chatsudthipong V.
spellingShingle Lungkaphin A.
Lewchalermwongse B.
Chatsudthipong V.
Relative contribution of OAT1 and OAT3 transport activities in isolated perfused rabbit renal proximal tubules
author_facet Lungkaphin A.
Lewchalermwongse B.
Chatsudthipong V.
author_sort Lungkaphin A.
title Relative contribution of OAT1 and OAT3 transport activities in isolated perfused rabbit renal proximal tubules
title_short Relative contribution of OAT1 and OAT3 transport activities in isolated perfused rabbit renal proximal tubules
title_full Relative contribution of OAT1 and OAT3 transport activities in isolated perfused rabbit renal proximal tubules
title_fullStr Relative contribution of OAT1 and OAT3 transport activities in isolated perfused rabbit renal proximal tubules
title_full_unstemmed Relative contribution of OAT1 and OAT3 transport activities in isolated perfused rabbit renal proximal tubules
title_sort relative contribution of oat1 and oat3 transport activities in isolated perfused rabbit renal proximal tubules
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/3179
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