Production of anti-dengue NS1 monoclonal antibodies by DNA immunization

• Main Authors: Puttikhunt C., Kasinrerk W., Srisa-ad S., Duangchinda T., Silakate W., Moonsom S., Sittisombut N., Malasit P.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-0037377570&partnerID=40&md5=76e9fb418cbde69b93176b44ef5a3cf2; http://cmuir.cmu.ac.th/handle/6653943832/3343
• Institution: Chiang Mai University

Monoclonal antibodies against dengue NS1 protein were generated following immunization of mice with plasmid DNA encoding the transmembrane form of NS1 from dengue serotype 2 virus. A mammalian expression vector, pDisplay, was engineered to direct cell surface expression of dengue NS1 and tested for...