High resolution DNA melting analysis: An application for prenatal control of α-thalassemia

• Main Authors: Sirichotiyakul S., Wanapirak C., Saetung R., Sanguansermsri T.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-77950674462&partnerID=40&md5=a34d3bd5654255586c89da2835e2a1a1; http://cmuir.cmu.ac.th/handle/6653943832/3672
• Institution: Chiang Mai University

Objective: To report the use of real-time gap-PCR using SYTO9 with high-resolution melting analysis (HRMA) in prenatal diagnosis of α-thalassemia 1. Materials and methods: Real-time gap-PCR using SYTO9 with HRMA was performed in 33 DNA samples from chorionic villi sampling (8 normal, 16 heterozygous...