Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a h...
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| التنسيق: | مقال |
| اللغة: | English |
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2014
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| الوصول للمادة أونلاين: | http://www.scopus.com/inward/record.url?eid=2-s2.0-77349109960&partnerID=40&md5=ea19d64ea0b43ab83b2a3162a146ffb5 http://cmuir.cmu.ac.th/handle/6653943832/3674 |
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| المؤسسة: | Chiang Mai University |
| اللغة: | English |
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th-cmuir.6653943832-36742014-08-30T02:35:11Z Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis Thanchomnang T. Intapan P.M. Lulitanond V. Sangmaneedet S. Chungpivat S. Taweethavonsawat P. Choochote W. Maleewong W. A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a hybrid between an amplicon generated from the D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method were all 100%. Besides being highly sensitive and specific, this PCR is fast and offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring of D. immitis in mosquito vectors. © 2009 Elsevier B.V. All rights reserved. 2014-08-30T02:35:11Z 2014-08-30T02:35:11Z 2010 Article 3044017 10.1016/j.vetpar.2009.12.002 20045253 VPARD http://www.scopus.com/inward/record.url?eid=2-s2.0-77349109960&partnerID=40&md5=ea19d64ea0b43ab83b2a3162a146ffb5 http://cmuir.cmu.ac.th/handle/6653943832/3674 English |
| institution |
Chiang Mai University |
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Chiang Mai University Library |
| country |
Thailand |
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CMU Intellectual Repository |
| language |
English |
| description |
A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a hybrid between an amplicon generated from the D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method were all 100%. Besides being highly sensitive and specific, this PCR is fast and offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring of D. immitis in mosquito vectors. © 2009 Elsevier B.V. All rights reserved. |
| format |
Article |
| author |
Thanchomnang T. Intapan P.M. Lulitanond V. Sangmaneedet S. Chungpivat S. Taweethavonsawat P. Choochote W. Maleewong W. |
| spellingShingle |
Thanchomnang T. Intapan P.M. Lulitanond V. Sangmaneedet S. Chungpivat S. Taweethavonsawat P. Choochote W. Maleewong W. Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis |
| author_facet |
Thanchomnang T. Intapan P.M. Lulitanond V. Sangmaneedet S. Chungpivat S. Taweethavonsawat P. Choochote W. Maleewong W. |
| author_sort |
Thanchomnang T. |
| title |
Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis |
| title_short |
Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis |
| title_full |
Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis |
| title_fullStr |
Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis |
| title_full_unstemmed |
Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis |
| title_sort |
rapid detection of dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer pcr and melting curve analysis |
| publishDate |
2014 |
| url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-77349109960&partnerID=40&md5=ea19d64ea0b43ab83b2a3162a146ffb5 http://cmuir.cmu.ac.th/handle/6653943832/3674 |
| _version_ |
1681420093057138688 |