Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis

Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis

A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a h...

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Main Authors: Thanchomnang T., Intapan P.M., Lulitanond V., Sangmaneedet S., Chungpivat S., Taweethavonsawat P., Choochote W., Maleewong W.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-77349109960&partnerID=40&md5=ea19d64ea0b43ab83b2a3162a146ffb5
http://cmuir.cmu.ac.th/handle/6653943832/3674
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-36742014-08-30T02:35:11Z Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis Thanchomnang T. Intapan P.M. Lulitanond V. Sangmaneedet S. Chungpivat S. Taweethavonsawat P. Choochote W. Maleewong W. A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a hybrid between an amplicon generated from the D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method were all 100%. Besides being highly sensitive and specific, this PCR is fast and offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring of D. immitis in mosquito vectors. © 2009 Elsevier B.V. All rights reserved. 2014-08-30T02:35:11Z 2014-08-30T02:35:11Z 2010 Article 3044017 10.1016/j.vetpar.2009.12.002 20045253 VPARD http://www.scopus.com/inward/record.url?eid=2-s2.0-77349109960&partnerID=40&md5=ea19d64ea0b43ab83b2a3162a146ffb5 http://cmuir.cmu.ac.th/handle/6653943832/3674 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description A real-time fluorescence resonance energy transfer (FRET) PCR supplemented with melting curve analysis for the rapid molecular detection of Dirofilaria immitis in mosquito vectors and dog blood samples was developed. This real-time FRET PCR was based on the fluorescence melting curve analysis of a hybrid between an amplicon generated from the D. immitis ribosomal RNA gene sequence and specific fluorophore-labeled probes. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method were all 100%. Besides being highly sensitive and specific, this PCR is fast and offers a high throughput. Therefore it is a suitable and powerful tool for the diagnosis and for epidemiological surveys of canine dirofilariasis as well as for molecular xenomonitoring of D. immitis in mosquito vectors. © 2009 Elsevier B.V. All rights reserved.
format Article
author Thanchomnang T.
Intapan P.M.
Lulitanond V.
Sangmaneedet S.
Chungpivat S.
Taweethavonsawat P.
Choochote W.
Maleewong W.
spellingShingle Thanchomnang T.
Intapan P.M.
Lulitanond V.
Sangmaneedet S.
Chungpivat S.
Taweethavonsawat P.
Choochote W.
Maleewong W.
Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
author_facet Thanchomnang T.
Intapan P.M.
Lulitanond V.
Sangmaneedet S.
Chungpivat S.
Taweethavonsawat P.
Choochote W.
Maleewong W.
author_sort Thanchomnang T.
title Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
title_short Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
title_full Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
title_fullStr Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
title_full_unstemmed Rapid detection of Dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer PCR and melting curve analysis
title_sort rapid detection of dirofilaria immitis in mosquito vectors and dogs using a real-time fluorescence resonance energy transfer pcr and melting curve analysis
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-77349109960&partnerID=40&md5=ea19d64ea0b43ab83b2a3162a146ffb5
http://cmuir.cmu.ac.th/handle/6653943832/3674
_version_ 1681420093057138688

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