Quantification of hemoglobin Constant Spring in heterozygote and homozygote by a capillary electrophoresis method
Introduction: Capillary electrophoresis (CE) is a high-resolution method for detection of hemoglobin Constant Spring (Hb CS). Methods: The levels of Hb CS quantified by CE were compared among three groups of samples including heterozygote and homozygote of Hb CS as well as Hb H-CS disease classified...
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| Main Authors: | , , , , |
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| Format: | Article |
| Published: |
Wiley-Blackwell
2015
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| Subjects: | |
| Online Access: | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84857441219&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38097 |
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| Institution: | Chiang Mai University |
| Summary: | Introduction: Capillary electrophoresis (CE) is a high-resolution method for detection of hemoglobin Constant Spring (Hb CS). Methods: The levels of Hb CS quantified by CE were compared among three groups of samples including heterozygote and homozygote of Hb CS as well as Hb H-CS disease classified by DNA molecular diagnosis. The mean corpuscular volume (MCV) of red blood cells was also analyzed among these three groups. Results: Mean±SD of Hb CS level of the homozygote was not significantly different from that of the Hb H-CS disease (1.9±1.8 vs. 2.8±1.3, P=0.13), but it was significantly higher than that of the heterozygote (1.9±1.8 vs. 0.4±0.2, P=0.007). The MCV <70fL was found in Hb H-CS disease only. Conclusion: CE is the preferable method for screening of heterozygote and homozygote of Hb CS. Moreover, in conjunction with a lower MCV (<70fL), this approach provided a high resolution to identify Hb H-CS disease. © 2011 Blackwell Publishing Ltd. |
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