Quantification of hemoglobin Constant Spring in heterozygote and homozygote by a capillary electrophoresis method

• Main Authors: Pornprasert,S., Panyasai,S., Waneesorn,J., Kongthai,K., Singboottra,P.
• Format: Article
• Published: Wiley-Blackwell 2015
• Subjects: Clinical Biochemistry; Hematology; Biochemistry (medical)
• Online Access: http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84857441219&origin=inward; http://cmuir.cmu.ac.th/handle/6653943832/38097
• Institution: Chiang Mai University

Introduction: Capillary electrophoresis (CE) is a high-resolution method for detection of hemoglobin Constant Spring (Hb CS). Methods: The levels of Hb CS quantified by CE were compared among three groups of samples including heterozygote and homozygote of Hb CS as well as Hb H-CS disease classified by DNA molecular diagnosis. The mean corpuscular volume (MCV) of red blood cells was also analyzed among these three groups. Results: Mean±SD of Hb CS level of the homozygote was not significantly different from that of the Hb H-CS disease (1.9±1.8 vs. 2.8±1.3, P=0.13), but it was significantly higher than that of the heterozygote (1.9±1.8 vs. 0.4±0.2, P=0.007). The MCV <70fL was found in Hb H-CS disease only. Conclusion: CE is the preferable method for screening of heterozygote and homozygote of Hb CS. Moreover, in conjunction with a lower MCV (<70fL), this approach provided a high resolution to identify Hb H-CS disease. © 2011 Blackwell Publishing Ltd.