Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold

• Main Authors: Klangjorhor J., Nimkingratana P., Settakorn J., Pruksakorn D., Leerapun T., Arpornchayanon O., Rojanasthien S., Kongtawelert P., Pothacharoen P.
• Format: Article
• Published: BioMed Central 2015
• Subjects: Orthopedics and Sports Medicine; Surgery
• Online Access: http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84871103802&origin=inward; http://cmuir.cmu.ac.th/handle/6653943832/38231
• Institution: Chiang Mai University

Background: Autologous chondrocyte transplantation is a promising technique for treatment of cartilage defects. Three dimensional chondrocyte cultures on a scaffold are widely used to retain the chondrogenic phenotype. Using a biodegradable gelatin scaffold is one option for the cell delivery system, but molecular and histological studies of the method have not yet been done.Methods: We evaluated the chondrogenic property of the primary human chondrocyte on a gelatin scaffold as compared to a collagen scaffold over a period of 21 days. We examined the production of glycosaminoglycan by quantitative and histological analysis. Gene expression of cartilage-associated molecules was assessed by quantitative RT-PCR.Results: The gelatin scaffold showed the ability to promote chondrocyte expansion, chondrogenic phenotype retention at molecular and mRNA levels.Conclusions: This scaffold is thus suitable for use as an in vitro model for chondrocyte 3D culture. © 2012 Klangjorhor et al.; licensee BioMed Central Ltd.