Stigmalactam from Orophea enterocarpa induces human cancer cell apoptosis via a mitochondrial pathway
Stigmalactam, an aristolactam-type alkaloid extracted from Orophea enterocarpa, exerts cytotoxicity against several human and murine cancer cell lines, but the molecular mechanisms remain elusive. The aims of this study were to identify the mode and mechanisms of human cancer cell death induced by s...
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Asian Pacific Organization for Cancer Prevention
2015
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th-cmuir.6653943832-383712015-06-16T07:47:05Z Stigmalactam from Orophea enterocarpa induces human cancer cell apoptosis via a mitochondrial pathway Banjerdpongchai,R. Wudtiwai,B. Pompimon,W. Cancer Research Oncology Epidemiology Public Health, Environmental and Occupational Health Stigmalactam, an aristolactam-type alkaloid extracted from Orophea enterocarpa, exerts cytotoxicity against several human and murine cancer cell lines, but the molecular mechanisms remain elusive. The aims of this study were to identify the mode and mechanisms of human cancer cell death induced by stigmalactam employing human hepatocellular carcinoma HepG2 and human invasive breast cancer MDA-MB-231 cells as models, compared to normal murine fibroblasts. It was found that stigmalactam was toxic to HepG2 and MDA-MB-231 cells with IC50 levels of 23.0±2.67 μM and 33.2±4.54 μM, respectively, using MTT assays. At the same time the IC50 level towards murine normal fibroblast NIH3T3 cells was 24.4±6.75 μM. Reactive oxygen species (ROS) production was reduced in stigmalactam-treated cells dose dependently after 4 h of incubation, indicating antioxidant activity, measured by using 2',7',-dichlorohydrofluorescein diacetate and flow cytometry. Caspase-3 and caspase-9 activities were increased in a dose response manner, while stigmalactam decreased the mitochondrial transmembrane potential dose-dependently in HepG2 cells, using 3,3'-dihexyloxacarbocyanine iodide and flow cytometry, indicating mitochondrial pathway-mediated apoptosis. In conclusion, stigmalactam from O. enterocarpa was toxic to both HepG2 and MDA-MB-231 cells and induced human cancer HepG2 cells to undergo apoptosis via the intrinsic (mitochondrial) pathway. 2015-06-16T07:47:05Z 2015-06-16T07:47:05Z 2014-01-01 Article 15137368 2-s2.0-84921764192 10.7314/APJCP.2014.15.23.10397 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84921764192&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38371 Asian Pacific Organization for Cancer Prevention |
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Cancer Research Oncology Epidemiology Public Health, Environmental and Occupational Health Banjerdpongchai,R. Wudtiwai,B. Pompimon,W. Stigmalactam from Orophea enterocarpa induces human cancer cell apoptosis via a mitochondrial pathway |
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Stigmalactam, an aristolactam-type alkaloid extracted from Orophea enterocarpa, exerts cytotoxicity against several human and murine cancer cell lines, but the molecular mechanisms remain elusive. The aims of this study were to identify the mode and mechanisms of human cancer cell death induced by stigmalactam employing human hepatocellular carcinoma HepG2 and human invasive breast cancer MDA-MB-231 cells as models, compared to normal murine fibroblasts. It was found that stigmalactam was toxic to HepG2 and MDA-MB-231 cells with IC50 levels of 23.0±2.67 μM and 33.2±4.54 μM, respectively, using MTT assays. At the same time the IC50 level towards murine normal fibroblast NIH3T3 cells was 24.4±6.75 μM. Reactive oxygen species (ROS) production was reduced in stigmalactam-treated cells dose dependently after 4 h of incubation, indicating antioxidant activity, measured by using 2',7',-dichlorohydrofluorescein diacetate and flow cytometry. Caspase-3 and caspase-9 activities were increased in a dose response manner, while stigmalactam decreased the mitochondrial transmembrane potential dose-dependently in HepG2 cells, using 3,3'-dihexyloxacarbocyanine iodide and flow cytometry, indicating mitochondrial pathway-mediated apoptosis. In conclusion, stigmalactam from O. enterocarpa was toxic to both HepG2 and MDA-MB-231 cells and induced human cancer HepG2 cells to undergo apoptosis via the intrinsic (mitochondrial) pathway. |
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Article |
author |
Banjerdpongchai,R. Wudtiwai,B. Pompimon,W. |
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Banjerdpongchai,R. Wudtiwai,B. Pompimon,W. |
author_sort |
Banjerdpongchai,R. |
title |
Stigmalactam from Orophea enterocarpa induces human cancer cell apoptosis via a mitochondrial pathway |
title_short |
Stigmalactam from Orophea enterocarpa induces human cancer cell apoptosis via a mitochondrial pathway |
title_full |
Stigmalactam from Orophea enterocarpa induces human cancer cell apoptosis via a mitochondrial pathway |
title_fullStr |
Stigmalactam from Orophea enterocarpa induces human cancer cell apoptosis via a mitochondrial pathway |
title_full_unstemmed |
Stigmalactam from Orophea enterocarpa induces human cancer cell apoptosis via a mitochondrial pathway |
title_sort |
stigmalactam from orophea enterocarpa induces human cancer cell apoptosis via a mitochondrial pathway |
publisher |
Asian Pacific Organization for Cancer Prevention |
publishDate |
2015 |
url |
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84921764192&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38371 |
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1681421461532704768 |