In vitro immunostimulating activity of the dried sap from fermented thai rice on human and murine neutrophils

• Main Authors: Manosroi A., Lohcharoenkal W., Ruksiriwanich W., Kietthanakorn B., Manosroi W., Manosroi J.
• Format: Article
• Published: American Scientific Publishers 2015
• Subjects: Education; Health (social science); Mathematics (all); Energy (all); Computer Science (all); Environmental Science (all); Engineering (all)
• Online Access: http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84870918994&origin=inward; http://cmuir.cmu.ac.th/handle/6653943832/38530
• Institution: Chiang Mai University

The in vitro immunostimulating activity of the dried rice sap on human and murine neutrophils was investigated using nitroblue tetrazolium (NBT) dye reduction test. Thai Jasmine brown rice was fermented with 3 purified strains of yeasts (Candida krusii, S. cerevisiae and Candida sp.) and molds (Aspergillus sp., Mucor sp. and Rhizopus sp.). The obtained rice sap was dried by the 3 different methods including hot air drying (HAD), freeze drying (FD) and spray drying (SD). The dried rice sap from all drying processes showed the presence of ferulic acid and linoleic acid, while γ-oryzanol was founded only in the dried rice sap from the HAD method. All dried sap samples gave DPPH free radical scavenging and lipid peroxidation inhibition activity, but not metal ion chelating activity. The immunostimulatory activity of all dried rice sap samples both in human and murine neutrophils with %NBT positive cells was higher than the negative control in the range of 1.28 to 2.68 times. In murine neutrophils, there was no significant difference in immunostimulating activity of the dried rice sap prepared by different drying processes. However, in human neutrophils, the dried rice sap from the HAD process indicated significant higher immunostimulating activity than those from other drying processes and the positive control. Therefore, the dried rice sap by the HAD process which gave the highest antioxidant and immunomodulating activity can be further developed as the potential food supplements. © 2012 American Scientific Publishers. All rights reserved.