Isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane

© Penerbit Universiti Sains Malaysia, 2015. Endophytic nitrogen fixing bacteria were isolated from the leaves, stems and roots of industrial variety (cv. U-Thong 3; UT3), wild and chewing sugarcane plants grown for 6 weeks in nitrogen (N)-free sand. Eighty nine isolates of endophytic bacteria were o...

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Main Authors: Muangthong A., Youpensuk S., Rerkasem B.
Format: Article
Published: Universiti Sains Malaysia 2015
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Online Access:http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84929399530&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38961
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-389612015-06-16T07:54:42Z Isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane Muangthong A. Youpensuk S. Rerkasem B. Medicine (all) Biochemistry, Genetics and Molecular Biology (all) Agricultural and Biological Sciences (all) © Penerbit Universiti Sains Malaysia, 2015. Endophytic nitrogen fixing bacteria were isolated from the leaves, stems and roots of industrial variety (cv. U-Thong 3; UT3), wild and chewing sugarcane plants grown for 6 weeks in nitrogen (N)-free sand. Eighty nine isolates of endophytic bacteria were obtained on N-free agar. An acetylene reduction assay (ARA) detected nitrogenase activity in all 89 isolates. Three isolates from the chewing (C2HL2, C7HL1 and C34MR1) sugarcane and one isolate from the industrial sugarcane (UT3R1) varieties were characterised, and their responses to different yeast extract concentrations were investigated. Three different responses in nitrogenase activity were observed. Isolates C2HL2 and C7HL1 exhibited major increases with the addition of 0.005% yeast extract, C34MR1 exhibited no response, and UT3R1 exhibited a significant decrease in nitrogenase activity with 0.005% yeast extract. In all the isolates, nitrogenase activity decreased with further increase of the yeast extract to 0.05%. The highest nitrogenase activity was observed in isolates C2HL2 and C7HL1, which had 16S rRNA gene sequences that were closely related to Novosphingobium sediminicola and Ochrobactrum intermedium, respectively. 2015-06-16T07:54:42Z 2015-06-16T07:54:42Z 2015-01-01 Article 19853718 2-s2.0-84929399530 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84929399530&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/38961 Universiti Sains Malaysia
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine (all)
Biochemistry, Genetics and Molecular Biology (all)
Agricultural and Biological Sciences (all)
spellingShingle Medicine (all)
Biochemistry, Genetics and Molecular Biology (all)
Agricultural and Biological Sciences (all)
Muangthong A.
Youpensuk S.
Rerkasem B.
Isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane
description © Penerbit Universiti Sains Malaysia, 2015. Endophytic nitrogen fixing bacteria were isolated from the leaves, stems and roots of industrial variety (cv. U-Thong 3; UT3), wild and chewing sugarcane plants grown for 6 weeks in nitrogen (N)-free sand. Eighty nine isolates of endophytic bacteria were obtained on N-free agar. An acetylene reduction assay (ARA) detected nitrogenase activity in all 89 isolates. Three isolates from the chewing (C2HL2, C7HL1 and C34MR1) sugarcane and one isolate from the industrial sugarcane (UT3R1) varieties were characterised, and their responses to different yeast extract concentrations were investigated. Three different responses in nitrogenase activity were observed. Isolates C2HL2 and C7HL1 exhibited major increases with the addition of 0.005% yeast extract, C34MR1 exhibited no response, and UT3R1 exhibited a significant decrease in nitrogenase activity with 0.005% yeast extract. In all the isolates, nitrogenase activity decreased with further increase of the yeast extract to 0.05%. The highest nitrogenase activity was observed in isolates C2HL2 and C7HL1, which had 16S rRNA gene sequences that were closely related to Novosphingobium sediminicola and Ochrobactrum intermedium, respectively.
format Article
author Muangthong A.
Youpensuk S.
Rerkasem B.
author_facet Muangthong A.
Youpensuk S.
Rerkasem B.
author_sort Muangthong A.
title Isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane
title_short Isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane
title_full Isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane
title_fullStr Isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane
title_full_unstemmed Isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane
title_sort isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane
publisher Universiti Sains Malaysia
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84929399530&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38961
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