Production and properties of two collagenases from bacteria and their application for collagen extraction

Two collagenolytic protease (collagenase) producing bacteria, a Gram positive Bacillus cereus CNA1 and a Gram negative Klebsiella pneumoniae CNL3, were isolated under alkaline and acidic conditions, respectively. The production of collagenase by these two bacteria was optimized. Glycerol was the sui...

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Main Authors: Suphatharaprateep,W., Cheirsilp,B., Jongjareonrak,A.
Format: Article
Published: Elsevier 2015
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http://cmuir.cmu.ac.th/handle/6653943832/39196
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-391962015-06-16T08:17:47Z Production and properties of two collagenases from bacteria and their application for collagen extraction Suphatharaprateep,W. Cheirsilp,B. Jongjareonrak,A. Bioengineering Molecular Biology Biotechnology Two collagenolytic protease (collagenase) producing bacteria, a Gram positive Bacillus cereus CNA1 and a Gram negative Klebsiella pneumoniae CNL3, were isolated under alkaline and acidic conditions, respectively. The production of collagenase by these two bacteria was optimized. Glycerol was the suitable carbon source for collagenase production by both strains. The optimal initial pH values for collagenase production by CNA1 and CNL3 were 7.5 and 6.0, respectively, and the optimal temperature was 37°C for both strains. The maximum activity of the partially purified collagenase from CNA1 was at pH 7.0 and 45°C. Its pH and thermal stability were in the range of 6-8 and below 40°C, respectively. The maximum activity of the partially purified collagenase from CNL3 was at pH 6.0 and 40°C. Its pH and thermal stability were in the range of 5-7 and below 37°C, respectively. The collagenase from CNL3 was more stable at a low pH compared with that from CNA1. Collagenases from both strains were used to extract collagen from salmon fish skin. The use of collagenases from CNA1 and CNL3 combined with acid treatment yielded a high collagen extraction of 54.6% and 53.0%, of the fish skin dry weight, respectively. © 2011 Elsevier B.V. 2015-06-16T08:17:47Z 2015-06-16T08:17:47Z 2011-10-01 Article 18716784 2-s2.0-80052964680 10.1016/j.nbt.2011.04.003 21549225 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80052964680&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/39196 Elsevier
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Bioengineering
Molecular Biology
Biotechnology
spellingShingle Bioengineering
Molecular Biology
Biotechnology
Suphatharaprateep,W.
Cheirsilp,B.
Jongjareonrak,A.
Production and properties of two collagenases from bacteria and their application for collagen extraction
description Two collagenolytic protease (collagenase) producing bacteria, a Gram positive Bacillus cereus CNA1 and a Gram negative Klebsiella pneumoniae CNL3, were isolated under alkaline and acidic conditions, respectively. The production of collagenase by these two bacteria was optimized. Glycerol was the suitable carbon source for collagenase production by both strains. The optimal initial pH values for collagenase production by CNA1 and CNL3 were 7.5 and 6.0, respectively, and the optimal temperature was 37°C for both strains. The maximum activity of the partially purified collagenase from CNA1 was at pH 7.0 and 45°C. Its pH and thermal stability were in the range of 6-8 and below 40°C, respectively. The maximum activity of the partially purified collagenase from CNL3 was at pH 6.0 and 40°C. Its pH and thermal stability were in the range of 5-7 and below 37°C, respectively. The collagenase from CNL3 was more stable at a low pH compared with that from CNA1. Collagenases from both strains were used to extract collagen from salmon fish skin. The use of collagenases from CNA1 and CNL3 combined with acid treatment yielded a high collagen extraction of 54.6% and 53.0%, of the fish skin dry weight, respectively. © 2011 Elsevier B.V.
format Article
author Suphatharaprateep,W.
Cheirsilp,B.
Jongjareonrak,A.
author_facet Suphatharaprateep,W.
Cheirsilp,B.
Jongjareonrak,A.
author_sort Suphatharaprateep,W.
title Production and properties of two collagenases from bacteria and their application for collagen extraction
title_short Production and properties of two collagenases from bacteria and their application for collagen extraction
title_full Production and properties of two collagenases from bacteria and their application for collagen extraction
title_fullStr Production and properties of two collagenases from bacteria and their application for collagen extraction
title_full_unstemmed Production and properties of two collagenases from bacteria and their application for collagen extraction
title_sort production and properties of two collagenases from bacteria and their application for collagen extraction
publisher Elsevier
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80052964680&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/39196
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