β-carotene production by Sporobolomyces pararoseus TISTR5213 using crude glycerol as the sole carbon source

© 2015, Chiang Mai University. All rights reserved. Recycling crude glycerol obtained from biodiesel production process has challenges, particularly as this glycerol is not suitable for foods and cosmetics applications. Therefore, nine strains of red yeasts from the culture collection of the Thailan...

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Main Authors: Manowattana,A., Techapun,C., Seesuriyachan,P., Hanmoungjai,P., Chaiyaso,T.
Format: Article
Published: Chiang Mai University 2015
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http://cmuir.cmu.ac.th/handle/6653943832/39228
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-392282015-06-16T08:17:55Z β-carotene production by Sporobolomyces pararoseus TISTR5213 using crude glycerol as the sole carbon source Manowattana,A. Techapun,C. Seesuriyachan,P. Hanmoungjai,P. Chaiyaso,T. Physics and Astronomy (all) Chemistry (all) Materials Science (all) Mathematics (all) Biochemistry, Genetics and Molecular Biology (all) © 2015, Chiang Mai University. All rights reserved. Recycling crude glycerol obtained from biodiesel production process has challenges, particularly as this glycerol is not suitable for foods and cosmetics applications. Therefore, nine strains of red yeasts from the culture collection of the Thailand Institute of Scientific and Technological Research (TISTR) were screened for β-carotene production in the basal medium supplemented with either pure glycerol (BMP) or crude glycerol (BMC) as the sole carbon source. The results revealed that Sporobolomyces pararoseus TISTR5213 showed the maximum β-carotene production yield of 0.62±0.05 and 1.17±0.01 mg/L in BMP and BMC, respectively. Variable medium components of BMC were selected in accordance with the Plackett-Burman experimental design with only one factor of crude glycerol being significant. The optimal conditions for physical factors (pH and temperature levels) were then combined in further studies through the response surface methodology approach. A quadratic model was constructed by central composite design (CCD). Using this experimental design, the β-carotene production yield increased from 1.17 to 27.41 mg/L or about 23 times higher than non-optimized BMC. The optimal conditions to achieve the maximum β-carotene yield were; crude glycerol 55.04 g/L, initial pH at 5.63 and 24.01°C for 5 days. 2015-06-16T08:17:55Z 2015-06-16T08:17:55Z 2015-01-01 Article 01252526 2-s2.0-84922013840 http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84922013840&origin=inward http://cmuir.cmu.ac.th/handle/6653943832/39228 Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Physics and Astronomy (all)
Chemistry (all)
Materials Science (all)
Mathematics (all)
Biochemistry, Genetics and Molecular Biology (all)
spellingShingle Physics and Astronomy (all)
Chemistry (all)
Materials Science (all)
Mathematics (all)
Biochemistry, Genetics and Molecular Biology (all)
Manowattana,A.
Techapun,C.
Seesuriyachan,P.
Hanmoungjai,P.
Chaiyaso,T.
β-carotene production by Sporobolomyces pararoseus TISTR5213 using crude glycerol as the sole carbon source
description © 2015, Chiang Mai University. All rights reserved. Recycling crude glycerol obtained from biodiesel production process has challenges, particularly as this glycerol is not suitable for foods and cosmetics applications. Therefore, nine strains of red yeasts from the culture collection of the Thailand Institute of Scientific and Technological Research (TISTR) were screened for β-carotene production in the basal medium supplemented with either pure glycerol (BMP) or crude glycerol (BMC) as the sole carbon source. The results revealed that Sporobolomyces pararoseus TISTR5213 showed the maximum β-carotene production yield of 0.62±0.05 and 1.17±0.01 mg/L in BMP and BMC, respectively. Variable medium components of BMC were selected in accordance with the Plackett-Burman experimental design with only one factor of crude glycerol being significant. The optimal conditions for physical factors (pH and temperature levels) were then combined in further studies through the response surface methodology approach. A quadratic model was constructed by central composite design (CCD). Using this experimental design, the β-carotene production yield increased from 1.17 to 27.41 mg/L or about 23 times higher than non-optimized BMC. The optimal conditions to achieve the maximum β-carotene yield were; crude glycerol 55.04 g/L, initial pH at 5.63 and 24.01°C for 5 days.
format Article
author Manowattana,A.
Techapun,C.
Seesuriyachan,P.
Hanmoungjai,P.
Chaiyaso,T.
author_facet Manowattana,A.
Techapun,C.
Seesuriyachan,P.
Hanmoungjai,P.
Chaiyaso,T.
author_sort Manowattana,A.
title β-carotene production by Sporobolomyces pararoseus TISTR5213 using crude glycerol as the sole carbon source
title_short β-carotene production by Sporobolomyces pararoseus TISTR5213 using crude glycerol as the sole carbon source
title_full β-carotene production by Sporobolomyces pararoseus TISTR5213 using crude glycerol as the sole carbon source
title_fullStr β-carotene production by Sporobolomyces pararoseus TISTR5213 using crude glycerol as the sole carbon source
title_full_unstemmed β-carotene production by Sporobolomyces pararoseus TISTR5213 using crude glycerol as the sole carbon source
title_sort β-carotene production by sporobolomyces pararoseus tistr5213 using crude glycerol as the sole carbon source
publisher Chiang Mai University
publishDate 2015
url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84922013840&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/39228
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