Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms

© 2017, Chiang Mai University. All rights reserved. Four saccharification buffers, including acetate, citrate, phthalate and succinate buffers, were evaluated for their effects on both saccharification and fermentation of rice husk holocellulose. At a concentration of 30 mM, all buffer systems were...

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Main Authors: Nachaiwieng W., Kanpiengjai A., Watanabe T., Khanongnuch C.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018492364&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/40604
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spelling th-cmuir.6653943832-406042017-09-28T04:10:27Z Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms Nachaiwieng W. Kanpiengjai A. Watanabe T. Khanongnuch C. © 2017, Chiang Mai University. All rights reserved. Four saccharification buffers, including acetate, citrate, phthalate and succinate buffers, were evaluated for their effects on both saccharification and fermentation of rice husk holocellulose. At a concentration of 30 mM, all buffer systems were found to be suitable for saccharification by three commercial cellulolytic enzymes (Meicelase, Accellerase 1500 and Accellerase XC). However, at concentrations of 50-200 mM, citrate and acetate buffers partially inhibited the fermenting ability of Saccharomyces cerevisiase, Zymomonas mobilis, Kluyveromyces marxianus, Candida shehatae and Pichia stipitis. This was in contrast to phthalate and succinate buffers in which these organisms retained 100% relative fermenting ability within concentrations in the range of 50-500 mM. This is the first report indicating potential buffers appropriate for broad ethanol producing microorganisms in bioethanol fermentation, particularly for co-culture fermentation of cellulosic hydrolysate. 2017-09-28T04:10:27Z 2017-09-28T04:10:27Z 2 Journal 01252526 2-s2.0-85018492364 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018492364&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/40604
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2017, Chiang Mai University. All rights reserved. Four saccharification buffers, including acetate, citrate, phthalate and succinate buffers, were evaluated for their effects on both saccharification and fermentation of rice husk holocellulose. At a concentration of 30 mM, all buffer systems were found to be suitable for saccharification by three commercial cellulolytic enzymes (Meicelase, Accellerase 1500 and Accellerase XC). However, at concentrations of 50-200 mM, citrate and acetate buffers partially inhibited the fermenting ability of Saccharomyces cerevisiase, Zymomonas mobilis, Kluyveromyces marxianus, Candida shehatae and Pichia stipitis. This was in contrast to phthalate and succinate buffers in which these organisms retained 100% relative fermenting ability within concentrations in the range of 50-500 mM. This is the first report indicating potential buffers appropriate for broad ethanol producing microorganisms in bioethanol fermentation, particularly for co-culture fermentation of cellulosic hydrolysate.
format Journal
author Nachaiwieng W.
Kanpiengjai A.
Watanabe T.
Khanongnuch C.
spellingShingle Nachaiwieng W.
Kanpiengjai A.
Watanabe T.
Khanongnuch C.
Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
author_facet Nachaiwieng W.
Kanpiengjai A.
Watanabe T.
Khanongnuch C.
author_sort Nachaiwieng W.
title Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
title_short Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
title_full Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
title_fullStr Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
title_full_unstemmed Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
title_sort influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018492364&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/40604
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