Evaluation of cryoprotective potential of Jerusalem artichoke' inulin during freeze-drying and storage of Lactobacillus paracasei HII01

The preservation of synbiotic preparations is the most important task than the formulation, due to the stability issues. Many advanced techniques are employed to preserve and stabilize the viability of synbiotic formulas. The current study deals with the development of freeze-dried (FD) Lactobacillu...

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Main Authors: Yamamoto S., Pattananandecha T., Sirilun S., Sivamaruthi B., Peerajan S., Chaiyasut C.
Format: Journal
Published: 2017
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84990049981&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41593
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-415932017-09-28T04:22:14Z Evaluation of cryoprotective potential of Jerusalem artichoke' inulin during freeze-drying and storage of Lactobacillus paracasei HII01 Yamamoto S. Pattananandecha T. Sirilun S. Sivamaruthi B. Peerajan S. Chaiyasut C. The preservation of synbiotic preparations is the most important task than the formulation, due to the stability issues. Many advanced techniques are employed to preserve and stabilize the viability of synbiotic formulas. The current study deals with the development of freeze-dried (FD) Lactobacillus paracasei HII01 with selected commercial inulins (inulin GR, inulin SC, inulin FF), inulin extracted from Jerusalem artichoke (inulin JA), skim milk, maltodextrin #2, trehalose, and sorbitol as cryoprotectants and assessment of viability during intestinal transit and storage. The trehalose, inulin F F, and inulin JA retain the survivability of L. paracasei HII01 up to 46.1-79.5%, 19.0-51.0%, and 10.3-33.1%, respectively, whereas, the sorbitol showed least survival rate (1.4-12.7%) of L. paracasei HII01 and the FD-L. paracasei HII01 without any cryoprotectants had no viable cells. The FD-powders with the high survival of L. paracasei HII01 were selected to study the viability during gastrointestinal transit (Simulated gastric fluid, and simulated intestinal fluid of human). The FD-L. paracasei HII01 with inulins protects the survivability of L. paracasei HII01 during gastrointestinal transit more than other studied protectants. The results suggested that the storage of FD-L. paracasei HII01 with inulin JA at 4°C can retain ∼59.2% of the existence of live L. paracasei HII01 cells for 60 days than other tested commercially available inulins, while the samples stored at room temperature showed the drastic reduction in the viability. Collectively, the results suggested that the inulin JA can be used in freeze drying process of L. paracasei HII01 as a cryoprotectant, which effectively diminishes the degradation of probiotic cells during gastrointestinal transit and storage. 2017-09-28T04:22:14Z 2017-09-28T04:22:14Z 2016-09-01 Journal 09737510 2-s2.0-84990049981 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84990049981&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/41593
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description The preservation of synbiotic preparations is the most important task than the formulation, due to the stability issues. Many advanced techniques are employed to preserve and stabilize the viability of synbiotic formulas. The current study deals with the development of freeze-dried (FD) Lactobacillus paracasei HII01 with selected commercial inulins (inulin GR, inulin SC, inulin FF), inulin extracted from Jerusalem artichoke (inulin JA), skim milk, maltodextrin #2, trehalose, and sorbitol as cryoprotectants and assessment of viability during intestinal transit and storage. The trehalose, inulin F F, and inulin JA retain the survivability of L. paracasei HII01 up to 46.1-79.5%, 19.0-51.0%, and 10.3-33.1%, respectively, whereas, the sorbitol showed least survival rate (1.4-12.7%) of L. paracasei HII01 and the FD-L. paracasei HII01 without any cryoprotectants had no viable cells. The FD-powders with the high survival of L. paracasei HII01 were selected to study the viability during gastrointestinal transit (Simulated gastric fluid, and simulated intestinal fluid of human). The FD-L. paracasei HII01 with inulins protects the survivability of L. paracasei HII01 during gastrointestinal transit more than other studied protectants. The results suggested that the storage of FD-L. paracasei HII01 with inulin JA at 4°C can retain ∼59.2% of the existence of live L. paracasei HII01 cells for 60 days than other tested commercially available inulins, while the samples stored at room temperature showed the drastic reduction in the viability. Collectively, the results suggested that the inulin JA can be used in freeze drying process of L. paracasei HII01 as a cryoprotectant, which effectively diminishes the degradation of probiotic cells during gastrointestinal transit and storage.
format Journal
author Yamamoto S.
Pattananandecha T.
Sirilun S.
Sivamaruthi B.
Peerajan S.
Chaiyasut C.
spellingShingle Yamamoto S.
Pattananandecha T.
Sirilun S.
Sivamaruthi B.
Peerajan S.
Chaiyasut C.
Evaluation of cryoprotective potential of Jerusalem artichoke' inulin during freeze-drying and storage of Lactobacillus paracasei HII01
author_facet Yamamoto S.
Pattananandecha T.
Sirilun S.
Sivamaruthi B.
Peerajan S.
Chaiyasut C.
author_sort Yamamoto S.
title Evaluation of cryoprotective potential of Jerusalem artichoke' inulin during freeze-drying and storage of Lactobacillus paracasei HII01
title_short Evaluation of cryoprotective potential of Jerusalem artichoke' inulin during freeze-drying and storage of Lactobacillus paracasei HII01
title_full Evaluation of cryoprotective potential of Jerusalem artichoke' inulin during freeze-drying and storage of Lactobacillus paracasei HII01
title_fullStr Evaluation of cryoprotective potential of Jerusalem artichoke' inulin during freeze-drying and storage of Lactobacillus paracasei HII01
title_full_unstemmed Evaluation of cryoprotective potential of Jerusalem artichoke' inulin during freeze-drying and storage of Lactobacillus paracasei HII01
title_sort evaluation of cryoprotective potential of jerusalem artichoke' inulin during freeze-drying and storage of lactobacillus paracasei hii01
publishDate 2017
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84990049981&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/41593
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