Structural characterization of sulfated arabinans extracted from Cladophora glomerata Kützing and their macrophage activation
© 2016 Japan Society for Bioscience, Biotechnology, and Agrochemistry. Water-soluble sulfated heteropolysaccharides were extracted from Cladophora glomerata Kützing and fractionated by ion-exchange chromatography, which yielded two subfractions, F 1 and F 2 . The crude and fractionated polysacchari...
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| Main Authors: | , , , , , |
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| Format: | Journal |
| Published: |
2017
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| Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84964692651&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/42411 |
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| Institution: | Chiang Mai University |
| Summary: | © 2016 Japan Society for Bioscience, Biotechnology, and Agrochemistry. Water-soluble sulfated heteropolysaccharides were extracted from Cladophora glomerata Kützing and fractionated by ion-exchange chromatography, which yielded two subfractions, F 1 and F 2 . The crude and fractionated polysaccharides (F 1 and F 2 ) mostly consisted of carbohydrates (62.8-74.5%) with various amounts of proteins (9.00-17.3%) and sulfates (16.5-23.5%), including different levels of arabinose (41.7-54.4%), galactose (13.5-39.0%), glucose (0.80-10.6%), xylose (6.84-13.4%), and rhamnose (0.20-2.83%). Based on the size exclusion chromatography (SEC) profiles, the crude and fractions mainly contained one peak with shoulders having molecular weight (M w ) ranges of 358-1,501 × 10 3 . The F 1 fraction stimulated RAW264.7 cells to produce considerable amounts of nitric oxide and cytokines compared to the crude and F 2 fraction. The backbone of the most potent immunostimulating fraction (F 1 ) was α-(1→4)-L-arabinopy-ranoside with galactose and xylose residues as branches at O-2 position, and sulfates mainly at O-2 position as well. |
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