Chemical stability enhancement and cytotoxicity reduction of papain loaded in PLGA nanospheres

Poly(lactic-co-glycolic acid) (PLGA) nanospheres loaded with papain were prepared by the emulsion solvent diffusion in water (ESD) and the w/o/w emulsion solvent evaporation (ESE) methods. The nanosphere loaded with papain from the ESE method gave smaller particle sizes (220-232 nm) and higher encap...

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Main Authors: Charinya Chankhampan, Jiradej Manosroi, Hiromitsu Yamamoto, Kohei Tahara, Worapaka Manosroi, Yoshiaki Kawashima, Aranya Manosroi
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84891533280&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45185
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-451852018-01-24T06:06:27Z Chemical stability enhancement and cytotoxicity reduction of papain loaded in PLGA nanospheres Charinya Chankhampan Jiradej Manosroi Hiromitsu Yamamoto Kohei Tahara Worapaka Manosroi Yoshiaki Kawashima Aranya Manosroi Poly(lactic-co-glycolic acid) (PLGA) nanospheres loaded with papain were prepared by the emulsion solvent diffusion in water (ESD) and the w/o/w emulsion solvent evaporation (ESE) methods. The nanosphere loaded with papain from the ESE method gave smaller particle sizes (220-232 nm) and higher encapsulation efficiency of about two-folds than those from the ESD method. The morphology of the nanospheres loaded with papain prepared by the ESE method exhibited spherical shape and smooth surface investigated by SEM and TEM. The release profile of papain from the PLGA nanospheres of the ESD and ESE method indicated two phases with an initial rapid phase of 6 h and followed by the slow release phase of 48 h. The unloaded PLGA nanospheres from the two methods did not show any cytotoxicity in human skin fibroblasts, while the unloaded papain gave toxicity more than the loaded papain of 1.5 times. Papain loaded in PLGA nanospheres prepared by the ESE method was more chemical stable than the unloaded papain of eight and three times when kept at 4°C and 25°C for 6 weeks, respectively. The developed stable and low cytotoxic nanosphere loaded with papain can be further developed as topical products. © 2014 Taylor and Francis Group, LLC. 2018-01-24T06:06:27Z 2018-01-24T06:06:27Z 2014-02-01 Journal 17458099 17458080 2-s2.0-84891533280 10.1080/17458080.2011.636385 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84891533280&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/45185
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description Poly(lactic-co-glycolic acid) (PLGA) nanospheres loaded with papain were prepared by the emulsion solvent diffusion in water (ESD) and the w/o/w emulsion solvent evaporation (ESE) methods. The nanosphere loaded with papain from the ESE method gave smaller particle sizes (220-232 nm) and higher encapsulation efficiency of about two-folds than those from the ESD method. The morphology of the nanospheres loaded with papain prepared by the ESE method exhibited spherical shape and smooth surface investigated by SEM and TEM. The release profile of papain from the PLGA nanospheres of the ESD and ESE method indicated two phases with an initial rapid phase of 6 h and followed by the slow release phase of 48 h. The unloaded PLGA nanospheres from the two methods did not show any cytotoxicity in human skin fibroblasts, while the unloaded papain gave toxicity more than the loaded papain of 1.5 times. Papain loaded in PLGA nanospheres prepared by the ESE method was more chemical stable than the unloaded papain of eight and three times when kept at 4°C and 25°C for 6 weeks, respectively. The developed stable and low cytotoxic nanosphere loaded with papain can be further developed as topical products. © 2014 Taylor and Francis Group, LLC.
format Journal
author Charinya Chankhampan
Jiradej Manosroi
Hiromitsu Yamamoto
Kohei Tahara
Worapaka Manosroi
Yoshiaki Kawashima
Aranya Manosroi
spellingShingle Charinya Chankhampan
Jiradej Manosroi
Hiromitsu Yamamoto
Kohei Tahara
Worapaka Manosroi
Yoshiaki Kawashima
Aranya Manosroi
Chemical stability enhancement and cytotoxicity reduction of papain loaded in PLGA nanospheres
author_facet Charinya Chankhampan
Jiradej Manosroi
Hiromitsu Yamamoto
Kohei Tahara
Worapaka Manosroi
Yoshiaki Kawashima
Aranya Manosroi
author_sort Charinya Chankhampan
title Chemical stability enhancement and cytotoxicity reduction of papain loaded in PLGA nanospheres
title_short Chemical stability enhancement and cytotoxicity reduction of papain loaded in PLGA nanospheres
title_full Chemical stability enhancement and cytotoxicity reduction of papain loaded in PLGA nanospheres
title_fullStr Chemical stability enhancement and cytotoxicity reduction of papain loaded in PLGA nanospheres
title_full_unstemmed Chemical stability enhancement and cytotoxicity reduction of papain loaded in PLGA nanospheres
title_sort chemical stability enhancement and cytotoxicity reduction of papain loaded in plga nanospheres
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84891533280&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45185
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