Exopolysaccharides from lactic acid bacteria: Structural analysis, molecular weight effect on immunomodulation

• Main Authors: Utoomporn Surayot, Jianguo Wang, Phisit Seesuriyachan, Ampin Kuntiya, Mehdi Tabarsa, Yong Jin Lee, Jin Kyung Kim, Woo Jung Park, Sang Guan You
• Format: Journal
• Published: 2018
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84900990109&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/45778
• Institution: Chiang Mai University

Exopolysaccharides (EPS) obtained from the culture medium of Lactobacillus confusus TISTR 1498 were investigated to determine their molecular characteristics and the effect of molecular weight (M w ) on immunomodulatory activity. The EPS mainly consisted of carbohydrates (81.9±2.4%) with only one type of monosaccharide, D-glucose, which was mostly connected by α-(1→6) glycosidic linkages. The EPS itself was unable to stimulate RAW264.7 cells to produce pro-inflammatory mediator nitric oxide (NO) and cytokines. However, considerable stimulation of RAW264.7 cells was observed by the low M w of EPSs having M w values≤70×10 3 g/mol. The partially hydrolyzed EPS stimulated RAW264.7 cells to induce considerable NO and various cytokine production such as TNF-α, IL-1β, IL-6 and IL-10 via up-regulation of their mRNA expression. In addition, the degradation Iκ-B and the phosphorylation of c-Jun NH 2 -terminal kinase (JNK) were facilitated by BW-30 and MW-40, suggesting that the partially hydrolyzed EPS stimulated RAW264.7 cells through the activation of NF-κB and JNK pathways. © 2014 Elsevier B.V.