Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1T strain from the Mariana Trench

This study reports the characterization of the ability of Dermacoccus spp. isolated from the deepest point of the world's oceans for azo dye decolorization. A detailed investigation of D ermacoccus abyssi MT1.1T with respect to the azoreductase activity and enzymatic mechanism as well as the po...

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Main Authors: Lang W., Sirisansaneeyakul S., Martins L.O., Ngiwsara L., Sakairi N., Pathom-aree W., Okuyama M., Mori H., Kimura A.
Format: Article
Language:English
Published: Academic Press 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84896940733&partnerID=40&md5=5ecda257c64b7336e876d1a9786c7d7f
http://cmuir.cmu.ac.th/handle/6653943832/4791
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-47912014-08-30T02:55:46Z Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1T strain from the Mariana Trench Lang W. Sirisansaneeyakul S. Martins L.O. Ngiwsara L. Sakairi N. Pathom-aree W. Okuyama M. Mori H. Kimura A. This study reports the characterization of the ability of Dermacoccus spp. isolated from the deepest point of the world's oceans for azo dye decolorization. A detailed investigation of D ermacoccus abyssi MT1.1T with respect to the azoreductase activity and enzymatic mechanism as well as the potential role of the bacterial strain for biocleaning of industrial dye baths is reported. Resting cells with oxygen-insensitive azoreductase resulted in the rapid decolorization of the polysulfonated dye Brilliant Black BN (BBN) which is a common food colorant. The highest specific decolorization rate (vs) was found at 50°C with a moderately thermal tolerance for over 1h. Kinetic analysis showed the high rates and strong affinity of the enzymatic system for the dye with a Vmax=137mg/gcell/h and a Km=19mg/L. The degradation of BBN produces an initial orange intermediate, 8-amino-5-((4-sulfonatophenyl)diazenyl)naphthalene-2-sulfonic acid, identified by mass spectrometry which is later converted to 4-aminobenzene sulfonic acid. Nearly 80% of the maximum vs is possible achieved in resting cell treatment with the salinity increased up to 5.0% NaCl in reaction media. Therefore, this bacterial system has potential for dye decolorization bioprocesses occurring at high temperature and salt concentrations e.g. for cleaning dye-containing saline wastewaters. © 2013 Elsevier Ltd. 2014-08-30T02:55:46Z 2014-08-30T02:55:46Z 2014 Article 10958630 10.1016/j.jenvman.2013.11.002 JEVMA http://www.scopus.com/inward/record.url?eid=2-s2.0-84896940733&partnerID=40&md5=5ecda257c64b7336e876d1a9786c7d7f http://cmuir.cmu.ac.th/handle/6653943832/4791 English Academic Press
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description This study reports the characterization of the ability of Dermacoccus spp. isolated from the deepest point of the world's oceans for azo dye decolorization. A detailed investigation of D ermacoccus abyssi MT1.1T with respect to the azoreductase activity and enzymatic mechanism as well as the potential role of the bacterial strain for biocleaning of industrial dye baths is reported. Resting cells with oxygen-insensitive azoreductase resulted in the rapid decolorization of the polysulfonated dye Brilliant Black BN (BBN) which is a common food colorant. The highest specific decolorization rate (vs) was found at 50°C with a moderately thermal tolerance for over 1h. Kinetic analysis showed the high rates and strong affinity of the enzymatic system for the dye with a Vmax=137mg/gcell/h and a Km=19mg/L. The degradation of BBN produces an initial orange intermediate, 8-amino-5-((4-sulfonatophenyl)diazenyl)naphthalene-2-sulfonic acid, identified by mass spectrometry which is later converted to 4-aminobenzene sulfonic acid. Nearly 80% of the maximum vs is possible achieved in resting cell treatment with the salinity increased up to 5.0% NaCl in reaction media. Therefore, this bacterial system has potential for dye decolorization bioprocesses occurring at high temperature and salt concentrations e.g. for cleaning dye-containing saline wastewaters. © 2013 Elsevier Ltd.
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author Lang W.
Sirisansaneeyakul S.
Martins L.O.
Ngiwsara L.
Sakairi N.
Pathom-aree W.
Okuyama M.
Mori H.
Kimura A.
spellingShingle Lang W.
Sirisansaneeyakul S.
Martins L.O.
Ngiwsara L.
Sakairi N.
Pathom-aree W.
Okuyama M.
Mori H.
Kimura A.
Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1T strain from the Mariana Trench
author_facet Lang W.
Sirisansaneeyakul S.
Martins L.O.
Ngiwsara L.
Sakairi N.
Pathom-aree W.
Okuyama M.
Mori H.
Kimura A.
author_sort Lang W.
title Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1T strain from the Mariana Trench
title_short Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1T strain from the Mariana Trench
title_full Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1T strain from the Mariana Trench
title_fullStr Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1T strain from the Mariana Trench
title_full_unstemmed Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1T strain from the Mariana Trench
title_sort biodecolorization of a food azo dye by the deep sea dermacoccus abyssi mt1.1t strain from the mariana trench
publisher Academic Press
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84896940733&partnerID=40&md5=5ecda257c64b7336e876d1a9786c7d7f
http://cmuir.cmu.ac.th/handle/6653943832/4791
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