A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model

Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to di...

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Bibliographic Details
Main Authors: Maslin Osathanunkul, Kittisak Buddhachat, Siriwadee Chomdej
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84870695160&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48106
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Institution: Chiang Mai University