A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model

A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model

Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to di...

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Main Authors: Maslin Osathanunkul, Kittisak Buddhachat, Siriwadee Chomdej
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84870695160&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48106
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-481062018-04-25T08:47:43Z A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model Maslin Osathanunkul Kittisak Buddhachat Siriwadee Chomdej Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to dissolve the dye and measurement of the absorbance at 600 nm was done to detect the level of gelatin (up to 10 μg), with the limit of detection for the amount of collagenase at 50 ng. This approach is rapid, simple, and economic for the purpose of screening for pharmaceutical agents that possess inhibitory activity on collagenase. © 2012 Elsevier Inc. All rights reserved. 2018-04-25T08:47:43Z 2018-04-25T08:47:43Z 2013-02-15 Journal 10960309 00032697 2-s2.0-84870695160 10.1016/j.ab.2012.09.036 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84870695160&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48106
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description Coomassie brilliant blue (CBB) heated by microwave was used as a staining dye for measuring gelatinolytic activity. The quantity of gelatin remaining after incubation with bacterial collagenase was determined using the heated CBB, resulting in visible blue pellets. Dimethyl sulfoxide was added to dissolve the dye and measurement of the absorbance at 600 nm was done to detect the level of gelatin (up to 10 μg), with the limit of detection for the amount of collagenase at 50 ng. This approach is rapid, simple, and economic for the purpose of screening for pharmaceutical agents that possess inhibitory activity on collagenase. © 2012 Elsevier Inc. All rights reserved.
format Journal
author Maslin Osathanunkul
Kittisak Buddhachat
Siriwadee Chomdej
spellingShingle Maslin Osathanunkul
Kittisak Buddhachat
Siriwadee Chomdej
A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
author_facet Maslin Osathanunkul
Kittisak Buddhachat
Siriwadee Chomdej
author_sort Maslin Osathanunkul
title A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_short A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_full A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_fullStr A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_full_unstemmed A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model
title_sort modified colorimetric method of gelatinolytic assay using bacterial collagenase type ii as a model
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84870695160&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48106
_version_ 1681423188056080384

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