Activity of immobilized papain dehydrated by n-propanol in low-water media
A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min-1 (mg powder)-1 in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4°C. Solid-state buffers (bases and their HCl salts) suspended in the organic m...
محفوظ في:
| المؤلفون الرئيسيون: | , , |
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| التنسيق: | مقال |
| اللغة: | English |
| منشور في: |
2014
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| الوصول للمادة أونلاين: | http://www.scopus.com/inward/record.url?eid=2-s2.0-1042268334&partnerID=40&md5=a11e2bb97ef6100a57c8d7cc4fa9adaf http://www.ncbi.nlm.nih.gov/pubmed/15000480 http://cmuir.cmu.ac.th/handle/6653943832/4844 |
| الوسوم: |
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| الملخص: | A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min-1 (mg powder)-1 in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4°C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium decreased the initial rate in all cases tested. The operational stability during Z-Gly-Phe-NH2 synthesis was improved when solid cysteine was added, doubling the yield after 24 h. |
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