Activity of immobilized papain dehydrated by n-propanol in low-water media
A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min-1 (mg powder)-1 in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4°C. Solid-state buffers (bases and their HCl salts) suspended in the organic m...
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2014
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th-cmuir.6653943832-48442014-08-30T02:55:51Z Activity of immobilized papain dehydrated by n-propanol in low-water media Theppakorn T. Kanasawud P. Halling P.J. A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min-1 (mg powder)-1 in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4°C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium decreased the initial rate in all cases tested. The operational stability during Z-Gly-Phe-NH2 synthesis was improved when solid cysteine was added, doubling the yield after 24 h. 2014-08-30T02:55:51Z 2014-08-30T02:55:51Z 2004 Article 01415492 10.1023/B:BILE.0000012891.13904.62 15000480 BILED http://www.scopus.com/inward/record.url?eid=2-s2.0-1042268334&partnerID=40&md5=a11e2bb97ef6100a57c8d7cc4fa9adaf http://www.ncbi.nlm.nih.gov/pubmed/15000480 http://cmuir.cmu.ac.th/handle/6653943832/4844 English |
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English |
| description |
A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min-1 (mg powder)-1 in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4°C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium decreased the initial rate in all cases tested. The operational stability during Z-Gly-Phe-NH2 synthesis was improved when solid cysteine was added, doubling the yield after 24 h. |
| format |
Article |
| author |
Theppakorn T. Kanasawud P. Halling P.J. |
| spellingShingle |
Theppakorn T. Kanasawud P. Halling P.J. Activity of immobilized papain dehydrated by n-propanol in low-water media |
| author_facet |
Theppakorn T. Kanasawud P. Halling P.J. |
| author_sort |
Theppakorn T. |
| title |
Activity of immobilized papain dehydrated by n-propanol in low-water media |
| title_short |
Activity of immobilized papain dehydrated by n-propanol in low-water media |
| title_full |
Activity of immobilized papain dehydrated by n-propanol in low-water media |
| title_fullStr |
Activity of immobilized papain dehydrated by n-propanol in low-water media |
| title_full_unstemmed |
Activity of immobilized papain dehydrated by n-propanol in low-water media |
| title_sort |
activity of immobilized papain dehydrated by n-propanol in low-water media |
| publishDate |
2014 |
| url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-1042268334&partnerID=40&md5=a11e2bb97ef6100a57c8d7cc4fa9adaf http://www.ncbi.nlm.nih.gov/pubmed/15000480 http://cmuir.cmu.ac.th/handle/6653943832/4844 |
| _version_ |
1681420313627197440 |