Activity of immobilized papain dehydrated by n-propanol in low-water media

• Main Authors: Theppakorn T., Kanasawud P., Halling P.J.
• 格式: Article
• 語言: English
• 出版: 2014
• 在線閱讀: http://www.scopus.com/inward/record.url?eid=2-s2.0-1042268334&partnerID=40&md5=a11e2bb97ef6100a57c8d7cc4fa9adaf; http://www.ncbi.nlm.nih.gov/pubmed/15000480; http://cmuir.cmu.ac.th/handle/6653943832/4844
• 機構: Chiang Mai University

A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min-1 (mg powder)-1 in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4°C. Solid-state buffers (bases and their HCl salts) suspended in the organic m...