Accumulation of lipid peroxidation-derived DNA lesions in iron-overloaded thalassemic mouse livers: Comparison with levels in the lymphocytes of thalassemia patients

In thalassemia patients, iron overload can stimulate lipid peroxidation (LPO), thereby generating miscoding DNA adducts. Adducted DNA was measured in the lymphocytes of β-Thal/Hb E patients and healthy controls and in the organs of thalassemic mice. εdA, εdC and M1dG residues were quantified by 32P-...

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Main Authors: Mayura Meerang, Jagadeesan Nair, Pornpan Sirankapracha, Chonthida Thephinlap, Somdet Srichairatanakool, Khelifa Arab, Ruchaneekorn Kalpravidh, Jim Vadolas, Suthat Fucharoen, Helmut Bartsch
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/48858
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-488582018-08-16T02:14:19Z Accumulation of lipid peroxidation-derived DNA lesions in iron-overloaded thalassemic mouse livers: Comparison with levels in the lymphocytes of thalassemia patients Mayura Meerang Jagadeesan Nair Pornpan Sirankapracha Chonthida Thephinlap Somdet Srichairatanakool Khelifa Arab Ruchaneekorn Kalpravidh Jim Vadolas Suthat Fucharoen Helmut Bartsch Biochemistry, Genetics and Molecular Biology Medicine In thalassemia patients, iron overload can stimulate lipid peroxidation (LPO), thereby generating miscoding DNA adducts. Adducted DNA was measured in the lymphocytes of β-Thal/Hb E patients and healthy controls and in the organs of thalassemic mice. εdA, εdC and M1dG residues were quantified by 32P-postlabeling-TLC/HPLC. M1dG levels in lymphocyte DNA from patients were 4 times as high as in controls, while the increase in εdA and εdC was not significant. Adducted DNA accumulated in the liver of thalassemic mice having >2.7 mg Fe/g tissue dry weight; DNA adducts and iron were highly correlated. edA was not specifically generated in certain mouse liver cell types as revealed by immunohistochemical staining. We found elevated LPO-induced DNA damage in the liver of thalassemic mouse and in lymphocytes, implicating that massive DNA damage occurs in the liver of thalassemia patients. We conclude that promutagenic LPO-derived DNA lesions are involved in the onset of hepatocellular carcinoma in these patients. © 2009 UICC. 2018-08-16T02:05:56Z 2018-08-16T02:05:56Z 2009-08-15 Journal 10970215 00207136 2-s2.0-67650065532 10.1002/ijc.24412 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650065532&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48858
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Mayura Meerang
Jagadeesan Nair
Pornpan Sirankapracha
Chonthida Thephinlap
Somdet Srichairatanakool
Khelifa Arab
Ruchaneekorn Kalpravidh
Jim Vadolas
Suthat Fucharoen
Helmut Bartsch
Accumulation of lipid peroxidation-derived DNA lesions in iron-overloaded thalassemic mouse livers: Comparison with levels in the lymphocytes of thalassemia patients
description In thalassemia patients, iron overload can stimulate lipid peroxidation (LPO), thereby generating miscoding DNA adducts. Adducted DNA was measured in the lymphocytes of β-Thal/Hb E patients and healthy controls and in the organs of thalassemic mice. εdA, εdC and M1dG residues were quantified by 32P-postlabeling-TLC/HPLC. M1dG levels in lymphocyte DNA from patients were 4 times as high as in controls, while the increase in εdA and εdC was not significant. Adducted DNA accumulated in the liver of thalassemic mice having >2.7 mg Fe/g tissue dry weight; DNA adducts and iron were highly correlated. edA was not specifically generated in certain mouse liver cell types as revealed by immunohistochemical staining. We found elevated LPO-induced DNA damage in the liver of thalassemic mouse and in lymphocytes, implicating that massive DNA damage occurs in the liver of thalassemia patients. We conclude that promutagenic LPO-derived DNA lesions are involved in the onset of hepatocellular carcinoma in these patients. © 2009 UICC.
format Journal
author Mayura Meerang
Jagadeesan Nair
Pornpan Sirankapracha
Chonthida Thephinlap
Somdet Srichairatanakool
Khelifa Arab
Ruchaneekorn Kalpravidh
Jim Vadolas
Suthat Fucharoen
Helmut Bartsch
author_facet Mayura Meerang
Jagadeesan Nair
Pornpan Sirankapracha
Chonthida Thephinlap
Somdet Srichairatanakool
Khelifa Arab
Ruchaneekorn Kalpravidh
Jim Vadolas
Suthat Fucharoen
Helmut Bartsch
author_sort Mayura Meerang
title Accumulation of lipid peroxidation-derived DNA lesions in iron-overloaded thalassemic mouse livers: Comparison with levels in the lymphocytes of thalassemia patients
title_short Accumulation of lipid peroxidation-derived DNA lesions in iron-overloaded thalassemic mouse livers: Comparison with levels in the lymphocytes of thalassemia patients
title_full Accumulation of lipid peroxidation-derived DNA lesions in iron-overloaded thalassemic mouse livers: Comparison with levels in the lymphocytes of thalassemia patients
title_fullStr Accumulation of lipid peroxidation-derived DNA lesions in iron-overloaded thalassemic mouse livers: Comparison with levels in the lymphocytes of thalassemia patients
title_full_unstemmed Accumulation of lipid peroxidation-derived DNA lesions in iron-overloaded thalassemic mouse livers: Comparison with levels in the lymphocytes of thalassemia patients
title_sort accumulation of lipid peroxidation-derived dna lesions in iron-overloaded thalassemic mouse livers: comparison with levels in the lymphocytes of thalassemia patients
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650065532&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48858
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