Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity

• Main Authors: Jiraporn Suwan, Zhenqing Zhang, Boyangzi Li, Preeyanat Vongchan, Puttinan Meepowpan, Fuming Zhang, Shaker A. Mousa, Shaymaa Mousa, Bhusana Premanode, Prachya Kongtawelert, Robert J. Linhardt
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=66649109421&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/48865
• Institution: Chiang Mai University

The novel low-molecular-weight chitosan polysulfate (MW 5120-26,200 Da) was prepared using the depolymerization of chitosan with papain (EC. 3.4.22.2). The sulfonation of depolymerized products was performed using chlorosulfonic acid in N,N-dimethylformamide under semi-heterogeneous conditions. The...