Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells

Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells

The aim of this study was to investigate the inhibitory mechanism of pure curcumin on WT1 expression in leukemic K562 cells. Pure curcumin suppressed WT1 expression, independent of effects on protein degradation or WT1 mRNA stability. Chromatin immunoprecipitation and reporter gene assays indicate t...

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Main Authors: Suwanna Semsri, Sheryl R. Krig, Lakmal Kotelawala, Colleen A. Sweeney, Songyot Anuchapreeda
Format: Journal
Published: 2018
Subjects:
Biochemistry, Genetics and Molecular Biology
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/49701
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-497012018-09-04T04:05:46Z Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells Suwanna Semsri Sheryl R. Krig Lakmal Kotelawala Colleen A. Sweeney Songyot Anuchapreeda Biochemistry, Genetics and Molecular Biology The aim of this study was to investigate the inhibitory mechanism of pure curcumin on WT1 expression in leukemic K562 cells. Pure curcumin suppressed WT1 expression, independent of effects on protein degradation or WT1 mRNA stability. Chromatin immunoprecipitation and reporter gene assays indicate that pure curcumin treatment attenuates WT1 auto-regulation. Interestingly, PKCα inhibition mimicks the repressive effects of pure curcumin in K562 cells. Conversely, myristoylated PKCα over-expression increased WT1 expression and reversed the inhibitory effect of pure curcumin. Our study indicates that pure curcumin attenuates WT1 auto-regulatory function through inhibition of PKCα signaling in K562 cells. © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. 2018-09-04T04:05:46Z 2018-09-04T04:05:46Z 2011-07-21 Journal 18733468 00145793 2-s2.0-79960383409 10.1016/j.febslet.2011.05.043 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79960383409&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49701
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Suwanna Semsri
Sheryl R. Krig
Lakmal Kotelawala
Colleen A. Sweeney
Songyot Anuchapreeda
Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
description The aim of this study was to investigate the inhibitory mechanism of pure curcumin on WT1 expression in leukemic K562 cells. Pure curcumin suppressed WT1 expression, independent of effects on protein degradation or WT1 mRNA stability. Chromatin immunoprecipitation and reporter gene assays indicate that pure curcumin treatment attenuates WT1 auto-regulation. Interestingly, PKCα inhibition mimicks the repressive effects of pure curcumin in K562 cells. Conversely, myristoylated PKCα over-expression increased WT1 expression and reversed the inhibitory effect of pure curcumin. Our study indicates that pure curcumin attenuates WT1 auto-regulatory function through inhibition of PKCα signaling in K562 cells. © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
format Journal
author Suwanna Semsri
Sheryl R. Krig
Lakmal Kotelawala
Colleen A. Sweeney
Songyot Anuchapreeda
author_facet Suwanna Semsri
Sheryl R. Krig
Lakmal Kotelawala
Colleen A. Sweeney
Songyot Anuchapreeda
author_sort Suwanna Semsri
title Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
title_short Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
title_full Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
title_fullStr Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
title_full_unstemmed Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
title_sort inhibitory mechanism of pure curcumin on wilms' tumor 1 (wt1) gene expression through the pkcα signaling pathway in leukemic k562 cells
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79960383409&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/49701
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