Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment

• Main Authors: Voravuth Somsak, Somdet Srichairatanakool, Sumalee Kamchonwongpaisan, Yongyuth Yuthavong, Chairat Uthaipibull
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79953903955&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/49713
• Institution: Chiang Mai University

The standard protocol for genetic modification of the rodent malaria parasite Plasmodium berghei requires infected blood from one or more laboratory mice, followed by large-scale in vitro parasite culture and purification of mature schizonts. Here, protocols are described for small-scale in vitro cu...