Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment
The standard protocol for genetic modification of the rodent malaria parasite Plasmodium berghei requires infected blood from one or more laboratory mice, followed by large-scale in vitro parasite culture and purification of mature schizonts. Here, protocols are described for small-scale in vitro cu...
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th-cmuir.6653943832-497132018-09-04T04:13:30Z Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment Voravuth Somsak Somdet Srichairatanakool Sumalee Kamchonwongpaisan Yongyuth Yuthavong Chairat Uthaipibull Biochemistry, Genetics and Molecular Biology Immunology and Microbiology The standard protocol for genetic modification of the rodent malaria parasite Plasmodium berghei requires infected blood from one or more laboratory mice, followed by large-scale in vitro parasite culture and purification of mature schizonts. Here, protocols are described for small-scale in vitro culture from 20 μL of mouse tail blood and purification of mature P. berghei schizonts sufficient for a single transfection experiment. All procedures are performed in 1.5-mL microcentrifuge tubes. We confirmed that transgenic parasites could be obtained using schizonts prepared by this protocol. This small-scale protocol provides significant advantages, namely reduction of parasite sample, laboratory consumables and mice for transfection experiments. © 2011 Elsevier B.V. All rights reserved. 2018-09-04T04:05:51Z 2018-09-04T04:05:51Z 2011-06-01 Journal 01666851 2-s2.0-79953903955 10.1016/j.molbiopara.2011.01.013 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79953903955&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49713 |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Voravuth Somsak Somdet Srichairatanakool Sumalee Kamchonwongpaisan Yongyuth Yuthavong Chairat Uthaipibull Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment |
| description |
The standard protocol for genetic modification of the rodent malaria parasite Plasmodium berghei requires infected blood from one or more laboratory mice, followed by large-scale in vitro parasite culture and purification of mature schizonts. Here, protocols are described for small-scale in vitro culture from 20 μL of mouse tail blood and purification of mature P. berghei schizonts sufficient for a single transfection experiment. All procedures are performed in 1.5-mL microcentrifuge tubes. We confirmed that transgenic parasites could be obtained using schizonts prepared by this protocol. This small-scale protocol provides significant advantages, namely reduction of parasite sample, laboratory consumables and mice for transfection experiments. © 2011 Elsevier B.V. All rights reserved. |
| format |
Journal |
| author |
Voravuth Somsak Somdet Srichairatanakool Sumalee Kamchonwongpaisan Yongyuth Yuthavong Chairat Uthaipibull |
| author_facet |
Voravuth Somsak Somdet Srichairatanakool Sumalee Kamchonwongpaisan Yongyuth Yuthavong Chairat Uthaipibull |
| author_sort |
Voravuth Somsak |
| title |
Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment |
| title_short |
Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment |
| title_full |
Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment |
| title_fullStr |
Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment |
| title_full_unstemmed |
Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment |
| title_sort |
small-scale in vitro culture and purification of plasmodium berghei for transfection experiment |
| publishDate |
2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79953903955&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49713 |
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