Human papilloma virus prevalence, genotype distribution, and pattern of infection in Thai women

Background: The pattern of infection in cervical lesions with respect to HPV subtype has not been systematically studied in Thai women. The aim here was to determine HPV prevalence, genotype, and infection pattern in cervical lesions and to estimate the potential efficacy of an HPV prophylactic vacc...

Full description

Saved in:
Bibliographic Details
Main Authors: Cheepsumon Suthipintawong, Sumalee Siriaunkgul, Kobkul Tungsinmunkong, Chamsai Pientong, Tipaya Ekalaksananan, Anant Karalak, Pilaiwan Kleebkaow, Songkhun Vinyuvat, Surang Triratanachat, Surapan Khunamornpong, Tuenjai Chongsuwanich
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84856284999&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/49771
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-49771
record_format dspace
spelling th-cmuir.6653943832-497712018-09-04T04:28:16Z Human papilloma virus prevalence, genotype distribution, and pattern of infection in Thai women Cheepsumon Suthipintawong Sumalee Siriaunkgul Kobkul Tungsinmunkong Chamsai Pientong Tipaya Ekalaksananan Anant Karalak Pilaiwan Kleebkaow Songkhun Vinyuvat Surang Triratanachat Surapan Khunamornpong Tuenjai Chongsuwanich Biochemistry, Genetics and Molecular Biology Medicine Background: The pattern of infection in cervical lesions with respect to HPV subtype has not been systematically studied in Thai women. The aim here was to determine HPV prevalence, genotype, and infection pattern in cervical lesions and to estimate the potential efficacy of an HPV prophylactic vaccine. Design: Formalinfixed paraffin-embedded cervical tissue blocks of 410 Thai patients from 8 institutes in 4 regions of Thailand (northern, southern, north-eastern, and central) were studied. The samples included 169 low grade squamous intraepithelial lesions (LSILs), 121 high grade squamous intraepithelial lesions (HSILs), and 120 squamous cell carcinomas (SCCs). HPV-DNA was amplified by PCR using consensus primers GP5+ and GP6+. The HPV genotype was then determined by reverse linear blot assay that included 37 HPV-specific 5'-amino-linked oligonucleotide probes. Patterns of infection were classified as single infection (one HPV type), double infection (two HPV types), and multiple infection (three or more HPV types). Results: The mean age of the subjects was 42 years. The prevalence of HPV infection was 88.8%. The highest HPV prevalence was found in the southern region (97.1%) and the lowest in the central region (78.6%). HPV-DNA was detected in 84.6% of LSILs, 90.1% of HSILs, and 93.3% of SCCs. A total of 20 HPV genotypes were identified. The five most common high risk HPV were HPV16 (83.2%), HPV18 (59.3%), HPV58 (9.3%), HPV52 (4.1%), and HPV45 (3.8%). In double and multiple infection patterns, the most common genotypes were HPV16/18 (27.8%) and HPV11/16/18 (54.9%). HPV6 was found only in LSIL and never in combination with other subtypes. HPV11 was most common in LSIL. Conclusion: There is no difference of HPV type distribution in women from 4 regions of Thailand with prominent HPV16 and HPV18 in all cases. The bivalent and quadrivalent vaccines have the potential to prevent 48.6 % and 74.5% of cervical cancers in Thai women. The potential of cancer prevention would rise to 87.6% if other frequent HR-HPV types (HPV58, 52, and 45) were also targeted by an HPV vaccine. 2018-09-04T04:17:51Z 2018-09-04T04:17:51Z 2011-01-01 Journal 2476762X 15137368 2-s2.0-84856284999 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84856284999&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49771
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Cheepsumon Suthipintawong
Sumalee Siriaunkgul
Kobkul Tungsinmunkong
Chamsai Pientong
Tipaya Ekalaksananan
Anant Karalak
Pilaiwan Kleebkaow
Songkhun Vinyuvat
Surang Triratanachat
Surapan Khunamornpong
Tuenjai Chongsuwanich
Human papilloma virus prevalence, genotype distribution, and pattern of infection in Thai women
description Background: The pattern of infection in cervical lesions with respect to HPV subtype has not been systematically studied in Thai women. The aim here was to determine HPV prevalence, genotype, and infection pattern in cervical lesions and to estimate the potential efficacy of an HPV prophylactic vaccine. Design: Formalinfixed paraffin-embedded cervical tissue blocks of 410 Thai patients from 8 institutes in 4 regions of Thailand (northern, southern, north-eastern, and central) were studied. The samples included 169 low grade squamous intraepithelial lesions (LSILs), 121 high grade squamous intraepithelial lesions (HSILs), and 120 squamous cell carcinomas (SCCs). HPV-DNA was amplified by PCR using consensus primers GP5+ and GP6+. The HPV genotype was then determined by reverse linear blot assay that included 37 HPV-specific 5'-amino-linked oligonucleotide probes. Patterns of infection were classified as single infection (one HPV type), double infection (two HPV types), and multiple infection (three or more HPV types). Results: The mean age of the subjects was 42 years. The prevalence of HPV infection was 88.8%. The highest HPV prevalence was found in the southern region (97.1%) and the lowest in the central region (78.6%). HPV-DNA was detected in 84.6% of LSILs, 90.1% of HSILs, and 93.3% of SCCs. A total of 20 HPV genotypes were identified. The five most common high risk HPV were HPV16 (83.2%), HPV18 (59.3%), HPV58 (9.3%), HPV52 (4.1%), and HPV45 (3.8%). In double and multiple infection patterns, the most common genotypes were HPV16/18 (27.8%) and HPV11/16/18 (54.9%). HPV6 was found only in LSIL and never in combination with other subtypes. HPV11 was most common in LSIL. Conclusion: There is no difference of HPV type distribution in women from 4 regions of Thailand with prominent HPV16 and HPV18 in all cases. The bivalent and quadrivalent vaccines have the potential to prevent 48.6 % and 74.5% of cervical cancers in Thai women. The potential of cancer prevention would rise to 87.6% if other frequent HR-HPV types (HPV58, 52, and 45) were also targeted by an HPV vaccine.
format Journal
author Cheepsumon Suthipintawong
Sumalee Siriaunkgul
Kobkul Tungsinmunkong
Chamsai Pientong
Tipaya Ekalaksananan
Anant Karalak
Pilaiwan Kleebkaow
Songkhun Vinyuvat
Surang Triratanachat
Surapan Khunamornpong
Tuenjai Chongsuwanich
author_facet Cheepsumon Suthipintawong
Sumalee Siriaunkgul
Kobkul Tungsinmunkong
Chamsai Pientong
Tipaya Ekalaksananan
Anant Karalak
Pilaiwan Kleebkaow
Songkhun Vinyuvat
Surang Triratanachat
Surapan Khunamornpong
Tuenjai Chongsuwanich
author_sort Cheepsumon Suthipintawong
title Human papilloma virus prevalence, genotype distribution, and pattern of infection in Thai women
title_short Human papilloma virus prevalence, genotype distribution, and pattern of infection in Thai women
title_full Human papilloma virus prevalence, genotype distribution, and pattern of infection in Thai women
title_fullStr Human papilloma virus prevalence, genotype distribution, and pattern of infection in Thai women
title_full_unstemmed Human papilloma virus prevalence, genotype distribution, and pattern of infection in Thai women
title_sort human papilloma virus prevalence, genotype distribution, and pattern of infection in thai women
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84856284999&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/49771
_version_ 1681423470026555392