Cellular uptake enhancement of Tat-GFP fusion protein loaded in elastic niosomes

This study has demonstrated the enhancement of cellular uptake of GFP when fused with Tat (Tat- GFP) and loaded in elastic niosomes. GFP and the GFP fused with Tat at C- and N-terminals were expressed in E. coli BL21 (DE3). The N-terminal Tat-GFP fusion protein (Tat-GFP) which showed the highest upt...

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Main Authors: Aranya Manosroi, Warangkana Lohcharoenkal, Friedrich Götz, Rolf G. Werner, Worapaka Manosroi, Jiradej Manosroi
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/49791
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-497912018-09-04T04:28:56Z Cellular uptake enhancement of Tat-GFP fusion protein loaded in elastic niosomes Aranya Manosroi Warangkana Lohcharoenkal Friedrich Götz Rolf G. Werner Worapaka Manosroi Jiradej Manosroi Chemical Engineering Engineering Materials Science Medicine Pharmacology, Toxicology and Pharmaceutics This study has demonstrated the enhancement of cellular uptake of GFP when fused with Tat (Tat- GFP) and loaded in elastic niosomes. GFP and the GFP fused with Tat at C- and N-terminals were expressed in E. coli BL21 (DE3). The N-terminal Tat-GFP fusion protein (Tat-GFP) which showed the highest uptake of 5.2% in HT-29 cell line at 2.4 folds of GFP was selected to load in various charged non-elastic and elastic niosomes and liposomes. All niosomes showed higher entrapment efficiency (EE) of the fusion protein more than in liposomes with the highest EE of 100% in elastic cationic niosomes. However, the fusion protein loaded in elastic anionic niosomes (Tween 61/cholesterol/dicetyl phosphate at 1:1:0.05 molar ratio) which gave the EE of only 32.8% showed the highest cellular uptake of GFP at 6.7, 2.8 and 1.7 times of GFP, Tat-GFP and Tat-GFP loaded in elastic cationic niosomes, respectively. After the 3 month-storage at 30±2 °C, the percentages remaining of the fusion protein loaded in the elastic anionic niosomes (61.9±2.7%) were about 2 times higher than the non-loaded fusion protein (33.7±2.8%). Thus, the cellular uptake and the chemical stability of the fusion protein were enhanced when loaded in elastic niosomes, especially the elastic anionic niosomes which can be further developed as an efficient delivery system for many therapeutic proteins. Copyright © 2011 American Scientific Publishers All rights reserved. 2018-09-04T04:18:08Z 2018-09-04T04:18:08Z 2011-06-01 Journal 15507041 15507033 2-s2.0-80052326914 10.1166/jbn.2011.1300 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80052326914&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49791
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemical Engineering
Engineering
Materials Science
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Chemical Engineering
Engineering
Materials Science
Medicine
Pharmacology, Toxicology and Pharmaceutics
Aranya Manosroi
Warangkana Lohcharoenkal
Friedrich Götz
Rolf G. Werner
Worapaka Manosroi
Jiradej Manosroi
Cellular uptake enhancement of Tat-GFP fusion protein loaded in elastic niosomes
description This study has demonstrated the enhancement of cellular uptake of GFP when fused with Tat (Tat- GFP) and loaded in elastic niosomes. GFP and the GFP fused with Tat at C- and N-terminals were expressed in E. coli BL21 (DE3). The N-terminal Tat-GFP fusion protein (Tat-GFP) which showed the highest uptake of 5.2% in HT-29 cell line at 2.4 folds of GFP was selected to load in various charged non-elastic and elastic niosomes and liposomes. All niosomes showed higher entrapment efficiency (EE) of the fusion protein more than in liposomes with the highest EE of 100% in elastic cationic niosomes. However, the fusion protein loaded in elastic anionic niosomes (Tween 61/cholesterol/dicetyl phosphate at 1:1:0.05 molar ratio) which gave the EE of only 32.8% showed the highest cellular uptake of GFP at 6.7, 2.8 and 1.7 times of GFP, Tat-GFP and Tat-GFP loaded in elastic cationic niosomes, respectively. After the 3 month-storage at 30±2 °C, the percentages remaining of the fusion protein loaded in the elastic anionic niosomes (61.9±2.7%) were about 2 times higher than the non-loaded fusion protein (33.7±2.8%). Thus, the cellular uptake and the chemical stability of the fusion protein were enhanced when loaded in elastic niosomes, especially the elastic anionic niosomes which can be further developed as an efficient delivery system for many therapeutic proteins. Copyright © 2011 American Scientific Publishers All rights reserved.
format Journal
author Aranya Manosroi
Warangkana Lohcharoenkal
Friedrich Götz
Rolf G. Werner
Worapaka Manosroi
Jiradej Manosroi
author_facet Aranya Manosroi
Warangkana Lohcharoenkal
Friedrich Götz
Rolf G. Werner
Worapaka Manosroi
Jiradej Manosroi
author_sort Aranya Manosroi
title Cellular uptake enhancement of Tat-GFP fusion protein loaded in elastic niosomes
title_short Cellular uptake enhancement of Tat-GFP fusion protein loaded in elastic niosomes
title_full Cellular uptake enhancement of Tat-GFP fusion protein loaded in elastic niosomes
title_fullStr Cellular uptake enhancement of Tat-GFP fusion protein loaded in elastic niosomes
title_full_unstemmed Cellular uptake enhancement of Tat-GFP fusion protein loaded in elastic niosomes
title_sort cellular uptake enhancement of tat-gfp fusion protein loaded in elastic niosomes
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80052326914&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/49791
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