Cellular uptake enhancement of Tat-GFP fusion protein loaded in elastic niosomes

• Main Authors: Aranya Manosroi, Warangkana Lohcharoenkal, Friedrich Götz, Rolf G. Werner, Worapaka Manosroi, Jiradej Manosroi
• Format: Journal
• Published: 2018
• Subjects: Chemical Engineering; Engineering; Materials Science; Medicine; Pharmacology, Toxicology and Pharmaceutics
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80052326914&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/49791
• Institution: Chiang Mai University

This study has demonstrated the enhancement of cellular uptake of GFP when fused with Tat (Tat- GFP) and loaded in elastic niosomes. GFP and the GFP fused with Tat at C- and N-terminals were expressed in E. coli BL21 (DE3). The N-terminal Tat-GFP fusion protein (Tat-GFP) which showed the highest upt...