Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis

• Main Authors: Brian W. Biegler, Shirley X. Yan, Sterling B. Ortega, Deepani K. Tennakoon, Michael K. Racke, Nitin J. Karandikar
• Format: Journal
• Published: 2018
• Subjects: Immunology and Microbiology; Medicine; Neuroscience
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79955666022&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/50032
• Institution: Chiang Mai University

Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease. © 2011 Elsevier B.V.